<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/schedule13g" xmlns:com="http://www.sec.gov/edgar/common">
  <headerData>
    <submissionType>SCHEDULE 13G</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <!-- Field: Pseudo-Tag; ID: Name; Data: SciSparc Ltd. -->
          <cik>0001611746</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
  </headerData>
  <formData>
    <coverPageHeader>
      <securitiesClassTitle>Common Stock, $0.0001 par value per share</securitiesClassTitle>
      <eventDateRequiresFilingThisStatement>10/20/2025</eventDateRequiresFilingThisStatement>
      <issuerInfo>
        <issuerCik>0001789192</issuerCik>
        <issuerName>N2OFF, Inc.</issuerName>
        <issuerCusip>80512Q501</issuerCusip>
        <issuerPrincipalExecutiveOfficeAddress>
          <com:street1>HaPardes</com:street1>
          <com:street2>134 (Meshek Sander)</com:street2>
          <com:city>Neve Yarak</com:city>
          <com:stateOrCountry>L3</com:stateOrCountry>
          <com:zipCode>4994500</com:zipCode>
        </issuerPrincipalExecutiveOfficeAddress>
      </issuerInfo>
      <designateRulesPursuantThisScheduleFiled>
        <designateRulePursuantThisScheduleFiled>Rule 13d-1(c)</designateRulePursuantThisScheduleFiled>
      </designateRulesPursuantThisScheduleFiled>
    </coverPageHeader>
    <coverPageHeaderReportingPersonDetails>


      <reportingPersonName>SciSparc Ltd.</reportingPersonName>
      <citizenshipOrOrganization>L3</citizenshipOrOrganization>
      <reportingPersonBeneficiallyOwnedNumberOfShares>
        <soleVotingPower>490751.00</soleVotingPower>
        <sharedVotingPower>0.00</sharedVotingPower>
        <soleDispositivePower>490751.00</soleDispositivePower>
        <sharedDispositivePower>0.00</sharedDispositivePower>
      </reportingPersonBeneficiallyOwnedNumberOfShares>
      <reportingPersonBeneficiallyOwnedAggregateNumberOfShares>490751.00</reportingPersonBeneficiallyOwnedAggregateNumberOfShares>
      <aggregateAmountExcludesCertainSharesFlag>N</aggregateAmountExcludesCertainSharesFlag>
      <classPercent>18.3</classPercent>
      <typeOfReportingPerson>CO</typeOfReportingPerson>
      <comments>(1) Consists of 490,751 Common Stock of the issuer held directly by SciSparc Ltd.

(2) Based on 2,682,483 Common Stock issued and outstanding as of October 20, 2025, based on information received from the Issuer.</comments>
    </coverPageHeaderReportingPersonDetails>
    <items>
      <item1>
        <issuerName>N2OFF, Inc.</issuerName>
        <issuerPrincipalExecutiveOfficeAddress>HaPardes 134 (Meshek Sander), Neve Yarak, Israel, 4994500.</issuerPrincipalExecutiveOfficeAddress>
      </item1>
      <item2>
        <filingPersonName>SciSparc Ltd.</filingPersonName>
        <principalBusinessOfficeOrResidenceAddress>20 Raul Wallenberg Street, Tower A, Tel Aviv 6971916 Israel.</principalBusinessOfficeOrResidenceAddress>
        <citizenship>Israel</citizenship>
      </item2>
      <item3>
        <notApplicableFlag>Y</notApplicableFlag>
      </item3>
      <item4>
        <amountBeneficiallyOwned>See items 5-11 of the cover pages hereto for beneficial ownership, percentage of class and dispositive power of the Reporting Person, which is incorporated herein.</amountBeneficiallyOwned>
        <classPercent>See items 5-11 of the cover pages hereto for beneficial ownership, percentage of class and dispositive power of the Reporting Person, which is incorporated herein.</classPercent>
        <numberOfSharesPersonHas>
          <solePowerOrDirectToVote>See items 5-11 of the cover pages hereto for beneficial ownership, percentage of class and dispositive power of the Reporting Person, which is incorporated herein.</solePowerOrDirectToVote>
          <sharedPowerOrDirectToVote>See items 5-11 of the cover pages hereto for beneficial ownership, percentage of class and dispositive power of the Reporting Person, which is incorporated herein.</sharedPowerOrDirectToVote>
          <solePowerOrDirectToDispose>See items 5-11 of the cover pages hereto for beneficial ownership, percentage of class and dispositive power of the Reporting Person, which is incorporated herein.</solePowerOrDirectToDispose>
          <sharedPowerOrDirectToDispose>See items 5-11 of the cover pages hereto for beneficial ownership, percentage of class and dispositive power of the Reporting Person, which is incorporated herein.</sharedPowerOrDirectToDispose>
        </numberOfSharesPersonHas>
      </item4>
      <item5>
        <notApplicableFlag>Y</notApplicableFlag>
      </item5>
      <item6>
        <notApplicableFlag>Y</notApplicableFlag>
      </item6>
      <item7>
        <notApplicableFlag>Y</notApplicableFlag>
      </item7>
      <item8>
        <notApplicableFlag>Y</notApplicableFlag>
      </item8>
      <item9>
        <notApplicableFlag>Y</notApplicableFlag>
      </item9>
      <item10>
        <notApplicableFlag>N</notApplicableFlag>
        <certifications>By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11.</certifications>
      </item10>
    </items>
    <signatureInformation>
      <reportingPersonName>SciSparc Ltd.</reportingPersonName>
      <signatureDetails>
        <signature>/s/ Oz Adler</signature>
        <title>Oz Adler / Chief Executive Officer &amp; Chief Financial Officer</title>
        <date>10/24/2025</date>
      </signatureDetails>
    </signatureInformation>
  </formData>
</edgarSubmission>
