<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001783036</cik>
        <entityName>NLS Pharmaceutics Ltd.</entityName>
        <issuerAddress>
            <street1>THE CIRCLE 6</street1>
            <city>ZURICH</city>
            <stateOrCountry>V8</stateOrCountry>
            <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
            <zipCode>CH-8058</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>+41.44.512.2150</issuerPhoneNumber>
        <jurisdictionOfInc>SWITZERLAND</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Other</entityType>
        <entityTypeOtherDesc>Limited Company</entityTypeOtherDesc>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Alexander</firstName>
                <lastName>Zwyer</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-8058</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chief Executive Officer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Ronald</firstName>
                <lastName>Hafner</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-8058</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Eric</firstName>
                <lastName>Konofal</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-8058</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chief Scientific Officer and Interim Chief Medical Officer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Elena</firstName>
                <lastName>Thyen-Pighin</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-8058</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chief Financial Officer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Gian-Marco</firstName>
                <lastName>Rinaldi</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-8058</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Audrey</firstName>
                <lastName>Greenberg</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-8058</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Claudio</firstName>
                <middleName>L.A.</middleName>
                <lastName>Basseti</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-8058</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Florence</firstName>
                <middleName>Allouche</middleName>
                <lastName>Aknin</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-8058</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Pharmaceuticals</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2024-06-28</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
            <isOptionToAcquireType>true</isOptionToAcquireType>
            <isSecurityToBeAcquiredType>true</isSecurityToBeAcquiredType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>H.C. Wainwright &amp; Co., LLC</recipientName>
                <recipientCRDNumber>000000375</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>430 Park Ave</street1>
                    <street2>3rd Floor</street2>
                    <city>New York</city>
                    <stateOrCountry>NY</stateOrCountry>
                    <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                    <zipCode>10022</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>NY</state>
                    <description>NEW YORK</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>786660</totalOfferingAmount>
            <totalAmountSold>786660</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse>Represents total gross proceeds received by the company in connection with a concurrent registered directoffering of common shares and concurrent private placement of warrants to purchase common shares.</clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>2</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>62933</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse>H.C. Wainwright &amp; Co., LLC (&quot;Wainwright&quot;) acted as the placement agent in connection with the offering. Wainwright received an aggregate fee equal to 8.0% of the gross proceeds raised in the offering and warrants to purchase up to 229,443 common shares.</clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>NLS Pharmaceutics Ltd.</issuerName>
                <signatureName>/s/ Alexander Zwyer</signatureName>
                <nameOfSigner>Alexander Zwyer</nameOfSigner>
                <signatureTitle>Chief Executive Officer</signatureTitle>
                <signatureDate>2024-07-12</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
