<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001783036</cik>
        <entityName>NLS Pharmaceutics Ltd.</entityName>
        <issuerAddress>
            <street1>THE CIRCLE 6</street1>
            <street2>8058</street2>
            <city>ZURICH</city>
            <stateOrCountry>V8</stateOrCountry>
            <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
            <zipCode>CH-6370</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>+41-44-512-2150</issuerPhoneNumber>
        <jurisdictionOfInc>SWITZERLAND</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Other</entityType>
        <entityTypeOtherDesc>Limited Company</entityTypeOtherDesc>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Alexander</firstName>
                <lastName>Zwyer</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <street2>8058</street2>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-6370</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chief Executive Officer and Director</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Chad</firstName>
                <lastName>Hellmann</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <street2>8058</street2>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-6370</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chief Financial Officer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Ronald</firstName>
                <lastName>Hafner</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <street2>8058</street2>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-6370</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chairman of the Board of Directors</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Myoung-Ok</firstName>
                <lastName>Kwon</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <street2>8058</street2>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-6370</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Stig Lokke</firstName>
                <lastName>Pedersen</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <street2>8058</street2>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-6370</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Gian-Marco</firstName>
                <lastName>Rinaldi de la Cruz</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>The Circle 6</street1>
                <street2>8058</street2>
                <city>Zurich</city>
                <stateOrCountry>V8</stateOrCountry>
                <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                <zipCode>CH-6370</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Pharmaceuticals</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2022-12-13</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
            <isOptionToAcquireType>true</isOptionToAcquireType>
            <isSecurityToBeAcquiredType>true</isSecurityToBeAcquiredType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>Laidlaw &amp; Company (UK) Ltd.</recipientName>
                <recipientCRDNumber>119037</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>521 5TH AVENUE</street1>
                    <street2>12 FLOOR</street2>
                    <city>NEW YORK</city>
                    <stateOrCountry>NY</stateOrCountry>
                    <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                    <zipCode>10175</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>CA</state>
                    <description>CALIFORNIA</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>30000000</totalOfferingAmount>
            <totalAmountSold>10000000</totalAmountSold>
            <totalRemaining>20000000</totalRemaining>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>4</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>700000</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>NLS Pharmaceutics Ltd.</issuerName>
                <signatureName>/s/ Alexander Zwyer</signatureName>
                <nameOfSigner>Alexander Zwyer</nameOfSigner>
                <signatureTitle>Chief Executive Officer and Director</signatureTitle>
                <signatureDate>2022-12-21</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
