X0609 4 2025-04-14 0001782223 Pyxis Oncology, Inc. PYXS 0001579781 HUMPHREY RACHEL false C/O PYXIS ONCOLOGY, INC. 321 HARRISON AVENUE, 11TH FL. SUITE 1 BOSTON MA 02118 true false false false true Common Stock 2025-04-14 4 S false 1400 0.9727 D 95405 D Common Stock 2025-04-17 4 S false 200 0.97 D 95205 D Common Stock 2025-04-21 4 S false 13896 1.0335 D 81309 D The sales reported in this Form 4 were effected pursuant to a Rule 10b5-1 trading plan adopted by the Reporting Person on December 5, 2024. Reflects sales of common stock executed in multiple transactions. The price reported reflects the weighted average sale price. The Reporting Person hereby undertakes to provide upon request by the Securities and Exchange Commission staff, the Issuer or a security holder of the Issuer full information regarding the number of shares and prices at which the sales were effected within the ranges set forth in footnotes 3 through 4 of this Form 4. The transaction was executed in multiple trades in prices ranging from $0.97 to $0.98. The transaction was executed in multiple trades in prices ranging from $0.97 to $1.09. /s/ Jitendra Wadhane, Attorney-in-Fact for Rachel Humphrey 2026-03-25