Exhibit 16.1

	AUDIT ALLIANCE LLP®
	A Top 18 Audit Firm
	10 Anson Road, #20-16 International Plaza, Singapore 079903.

UEN: T12LL1223B GST Reg No : M90367663E 

Tel: (65) 6227 5428 

10 Anson Road #20-16 International Plaza Singapore 079903

Website : www.allianceaudit.com

February 27, 2025

Securities and Exchange Commission

100 F Street, N.E.

Washington, D.C. 20549

Commissioners:

We have read Form 6-K dated February 27, 2025, of Mingzhu Logistics Holdings Limited. (“Registrant”) and are in agreement with the statements contained therein as it pertains to our firm; we are not in a position to agree or disagree with other statements of Registrant contained therein.

Very truly yours,

/s/ Audit Alliance LLP

Singapore

Registered Office: 10 Anson Road #20-16 International Plaza Singapore 079903