X0202 SCHEDULE 13G/A 0000935836-25-000708 0001105806 XXXXXXXX LIVE 1 Common Stock 06/30/2026 0001781983 Aprea Therapeutics, Inc. 03836J201 3805 OLD EASTON ROAD DOYLESTOWN PA 18902 Rule 13d-1(c) LYTTON LAURENCE W X1 322220.00 68587.00 322220.00 68587.00 390807.00 N 3.2 IN The percentage reported herein is calculated based on 12,382,776 shares of Common Stock outstanding as of May 13, 2026, as reported in the Form 10-Q filed by the issuer for the quarter ended March 31, 2026. Aprea Therapeutics, Inc. 3805 Old Easton Road, Doylestown, PA 18902 Laurence W. Lytton 467 CENTRAL PARK WEST, NEW YORK, NY 10025 United States Y 390,807 3.2% 322,220 68,587 322,220 68,587 Y Y Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under § 240.14a-11. LYTTON LAURENCE W /s/ Laurence W. Lytton 08/13/2026