X0306 4 2021-01-19 0 0001761612 BICYCLE THERAPEUTICS plc BCYC 0001776938 Lee Kevin C/O BICYCLE THERAPEUTICS PLC B900, BABRAHAM RESEARCH CAMPUS CAMBRIDGE X0 CB22 3AT UNITED KINGDOM 1 1 0 0 Chief Executive Officer Ordinary Shares 2021-01-19 4 S 0 4529 29.1389 D 260556 D Ordinary Shares 2021-01-19 4 S 0 371 30.2193 D 260185 D Ordinary Shares 2021-01-19 4 S 0 100 31.09 D 260085 D Ordinary Shares 2021-01-20 4 S 0 734 31.3272 D 259351 D Ordinary Shares 2021-01-20 4 S 0 100 32.02 D 259251 D Ordinary Shares 2021-01-20 4 S 0 351 33.00 D 258900 D Shares sold pursuant to a Rule 10b5-1 trading plan. The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $29.00 to $29.94 inclusive. The Reporting Person undertakes to provide to the Issuer, any security holder of the Issuer, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the range set forth in this footnote. The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $30.00 to $30.60 inclusive. The Reporting Person undertakes to provide to the Issuer, any security holder of the Issuer, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the range set forth in this footnote. The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $31.00 to $31.88 inclusive. The Reporting Person undertakes to provide to the Issuer, any security holder of the Issuer, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the range set forth in this footnote. /s/ Lee Kalowski, Attorney-in-Fact 2021-01-21