<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001761612</cik>
        <entityName>BICYCLE THERAPEUTICS PLC</entityName>
        <issuerAddress>
            <street1>BLOCKS A &amp; B, PORTWAY BUILDING</street1>
            <street2>GRANTA PARK, GREAT ABINGTON</street2>
            <city>CAMBRIDGE</city>
            <stateOrCountry>X0</stateOrCountry>
            <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
            <zipCode>CB21 6GS</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>011441223261503</issuerPhoneNumber>
        <jurisdictionOfInc>UNITED KINGDOM</jurisdictionOfInc>
        <edgarPreviousNameList>
            <previousName>BICYCLE THERAPEUTICS plc</previousName>
            <previousName>BICYCLE THERAPEUTICS Ltd</previousName>
        </edgarPreviousNameList>
        <entityType>Other</entityType>
        <entityTypeOtherDesc>PUBLIC LIMITED COMPANY</entityTypeOtherDesc>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>KEVIN</firstName>
                <lastName>LEE</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>ALETHIA</firstName>
                <lastName>YOUNG</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>PIERRE</firstName>
                <lastName>LEGAULT</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>FELIX</firstName>
                <middleName>J.</middleName>
                <lastName>BAKER</lastName>
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            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>JANICE</firstName>
                <lastName>BOURQUE</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
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            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>JOSE-CARLOS</firstName>
                <lastName>GUTIERREZ-RAMOS</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
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            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>RICHARD</firstName>
                <middleName>N.</middleName>
                <lastName>KENDER</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>STEPHEN</firstName>
                <lastName>SANDS</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
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                <relationship>Director</relationship>
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            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>GREGORY</firstName>
                <lastName>WINTER</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>SANTIAGO</firstName>
                <lastName>ARROYO</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>NIGEL</firstName>
                <lastName>CROCKETT</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>MICHAEL</firstName>
                <lastName>HANNAY</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>NICHOLAS</firstName>
                <lastName>KEEN</lastName>
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            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
                <stateOrCountry>X0</stateOrCountry>
                <stateOrCountryDescription>UNITED KINGDOM</stateOrCountryDescription>
                <zipCode>CB21 6GS</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>ALISTAIR</firstName>
                <lastName>MILNES</lastName>
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            <relatedPersonAddress>
                <street1>C/O BICYCLE THERAPEUTICS PLC</street1>
                <street2>BLOCKS A&amp;B PORTWAY BUILDING, GRANTA PARK</street2>
                <city>GREAT ABINGTON, CAMBRIDGE</city>
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                <zipCode>CB21 6GS</zipCode>
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            <relationshipClarification></relationshipClarification>
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        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>MICHAEL</firstName>
                <lastName>SKYNNER</lastName>
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                <relationship>Executive Officer</relationship>
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            <relationshipClarification></relationshipClarification>
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    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Pharmaceuticals</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2024-05-28</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>JEFFERIES LLC</recipientName>
                <recipientCRDNumber>2347</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>520 MADISON AVENUE</street1>
                    <city>NEW YORK</city>
                    <stateOrCountry>NY</stateOrCountry>
                    <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                    <zipCode>10022</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>CA</state>
                    <description>CALIFORNIA</description>
                    <state>CT</state>
                    <description>CONNECTICUT</description>
                    <state>MD</state>
                    <description>MARYLAND</description>
                    <state>MA</state>
                    <description>MASSACHUSETTS</description>
                    <state>MN</state>
                    <description>MINNESOTA</description>
                    <state>NY</state>
                    <description>NEW YORK</description>
                    <state>PA</state>
                    <description>PENNSYLVANIA</description>
                </statesOfSolicitationList>
                <foreignSolicitation>true</foreignSolicitation>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>555499983</totalOfferingAmount>
            <totalAmountSold>555499983</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>19</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>10110000</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>BICYCLE THERAPEUTICS PLC</issuerName>
                <signatureName>/s/ ALETHIA YOUNG</signatureName>
                <nameOfSigner>ALETHIA YOUNG</nameOfSigner>
                <signatureTitle>CHIEF FINANCIAL OFFICER</signatureTitle>
                <signatureDate>2024-06-11</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
