X0407 4 2023-03-31 0 0001761612 BICYCLE THERAPEUTICS plc BCYC 0001706857 Arroyo Santiago C/O BICYCLE THERAPEUTICS PLC BLOCKS A & B, PORTWAY BUILDING CAMBRIDGE X0 CB21 6GS UNITED KINGDOM 0 1 0 0 Chief Development Officer 0 Employee Stock Option (right to buy) 21.77 2023-03-31 4 A 0 120000 0.00 A 2033-03-31 Ordinary Shares 120000 120000 D This option shall vest with respect to one-fourth (1/4) of the total number of shares underlying the option on the first anniversary of the date of grant and the remaining shares shall vest in 36 equal monthly installments thereafter, subject to the individual remaining a service provider of the issuer through each such date. /s/ Lee Kalowski, Attorney-in-Fact 2023-04-04