X0206 3 2023-03-31 1 0001761612 BICYCLE THERAPEUTICS plc BCYC 0001706857 Arroyo Santiago C/O BICYCLE THERAPEUTICS PLC BLOCKS A & B, PORTWAY BUILDING CAMBRIDGE X0 CB21 6GS UNITED KINGDOM 0 1 0 0 Chief Development Officer No securities beneficially owned. Exhibit List - Exhibit 24 - Power of Attorney /s/ Lee Kalowski, Attorney-in-Fact 2023-04-04