SCHEDULE 13G/A 0000902664-24-006976 0001603466 XXXXXXXX LIVE 2 Ordinary Shares, nominal value 0.01 GBP per share 06/30/2025 0001761612 BICYCLE THERAPEUTICS PLC 088786108 BLOCKS A & B, PORTWAY BUILDING GRANTA PARK, GREAT ABINGTON CAMBRIDGE X0 CB21 6GS Rule 13d-1(c) Point72 Asset Management, L.P. b DE 0.00 1860317.00 0.00 1860317.00 1860317.00 N 3.9 PN Represented by 1,860,317 ADSs (as defined in Item 2(a)). Point72 Capital Advisors, Inc. b DE 0.00 1860317.00 0.00 1860317.00 1860317.00 N 3.9 CO Represented by 1,860,317 ADSs. Steven A. Cohen b X1 0.00 1860317.00 0.00 1860317.00 1860317.00 N 3.9 IN Represented by 1,860,317 ADSs. BICYCLE THERAPEUTICS PLC Blocks A & B, Portway Building Granta Park, Great Abington Cambridge United Kingdom CB21 6GS This statement is filed by: (i) Point72 Asset Management, L.P. ("Point72 Asset Management") with respect to the ordinary shares, nominal value 0.01 GBP per share ("Ordinary Shares"), of Bicycle Therapeutics plc held, in the form of American Depositary Shares ("ADSs"), by an investment fund it manages; (ii) Point72 Capital Advisors, Inc. ("Point72 Capital Advisors Inc.") with respect to Ordinary Shares held, in the form of ADSs, by an investment fund managed by Point72 Asset Management; and (iii) Steven A. Cohen ("Mr. Cohen") with respect to Ordinary Shares beneficially owned, in the form of ADSs, by Point72 Asset Management and Point72 Capital Advisors Inc. Cubist Systematic Strategies, LLC, an advisor under common control with Point72 Asset Management, acts as a sub-advisor with respect to a portion of the Ordinary Shares reported herein. There is no CUSIP number assigned to the Ordinary Shares. The CUSIP Number 088786108 has been assigned to the ADSs of the Issuer, which are quoted on the Nasdaq Global Select Market under the symbol "BCYC." Each ADS represents 1 Ordinary Share. The address of the principal business office of Point72 Asset Management, Point72 Capital Advisors Inc., and Mr. Cohen is 72 Cummings Point Road, Stamford, CT 06902. Point72 Asset Management is a Delaware limited partnership. Point72 Capital Advisors Inc. is a Delaware corporation. Mr. Cohen is a United States citizen. Y The information required by Item 4(a) is set forth in Row 9 of the cover page for each of the Reporting Persons and is incorporated herein by reference. Such information is as of the close of business on June 30, 2025. Point72 Asset Management, Point72 Capital Advisors Inc., and Mr. Cohen own directly no Ordinary Shares. Pursuant to an investment management agreement, Point72 Asset Management maintains investment and voting power with respect to the securities held by an investment fund it manages. Point72 Capital Advisors Inc. is the general partner of Point72 Asset Management. Mr. Cohen controls each of Point72 Asset Management and Point72 Capital Advisors Inc. The filing of this statement should not be construed as an admission that any of the foregoing persons or any Reporting Person is, for the purposes of Section 13 of the Securities and Exchange Act of 1934, the beneficial owner of the Ordinary Shares reported herein. 3.9% The information required by Item 4(c)(i) is set forth in Row 5 of the cover page for each of the Reporting Persons and is incorporated herein by reference. Such information is as of the close of business on June 30, 2025. The information required by Item 4(c)(ii) is set forth in Row 6 of the cover page for each of the Reporting Persons and is incorporated herein by reference. Such information is as of the close of business on June 30, 2025. The information required by Item 4(c)(iii) is set forth in Row 7 of the cover page for each of the Reporting Persons and is incorporated herein by reference. Such information is as of the close of business on June 30, 2025. The information required by Item 4(c)(iv) is set forth in Row 8 of the cover page for each of the Reporting Persons and is incorporated herein by reference. Such information is as of the close of business on June 30, 2025. N Y N See Item 2(a). Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. Point72 Asset Management, L.P. /s/ Jason M. Colombo 08/14/2025 Point72 Capital Advisors, Inc. /s/ Jason M. Colombo 08/14/2025 Steven A. Cohen /s/ Jason M. Colombo 08/14/2025