Exhibit 23.1

Consent of Independent Registered Public Accounting Firm

To the Board of Directors and Stockholders of Ascend Wellness Holdings, Inc.

We consent to the incorporation by reference in the Registration Statement on Form S-8 (File No. 333-257780) of Ascend Wellness Holdings, Inc. and Subsidiaries (the Company) of our report dated March 10, 2022, relating to the Company’s consolidated financial statements as of and for the year ended December 31, 2021, which report appears in this Annual Report on Form 10-K of Ascend Wellness Holdings, Inc. for the year ended December 31, 2021.

/s/ Macias Gini & O'Connell LLP

San Francisco, California

March 10, 2022