144 0001754464 XXXXXXXX LIVE 0001717115 TEMPUS AI, INC. 001-42130 600 WEST CHICAGO AVENUE, SUITE 510 CHICAGO IL 60654 800-976-5448 Ryan M. Bartolucci Officer Class A Common Stock Morgan Stanley Smith Barney LLC Executive Financial Services

1 New York Plaza, 8th Floor New York NY 10004

1904 149368.8 168683769 08/19/2025 NASDAQ Class A Common Stock 07/14/2021 Restricted Stock Units Issuer N 815 07/14/2021 N/A Class A Common Stock 04/27/2022 Restricted Stock Units Issuer N 350 04/27/2022 N/A Class A Common Stock 04/18/2023 Restricted Stock Units Issuer N 350 04/18/2023 N/A Class A Common Stock 05/02/2024 Restricted Stock Units Issuer N 389 05/02/2024 N/A N Ryan M. Bartolucci

C/O TEMPUS AI, INC. 600 WEST CHICAGO AVENUE, SUITE 510 CHICAGO IL 60654

Class A Common Stock 05/20/2025 6073 384022.79 The sales reported on this Form 144 are mandated by the Issuer's election under its equity incentive plan to require the satisfaction of a tax withholding obligation to be funded by sell to cover transactions and do not represent discretionary trades by the reporting person. 08/19/2025 /s/ Ryan M Bartolucci