SCHEDULE 13G 0001426156 XXXXXXXX LIVE Ordinary Shares, nominal value $0.000042 per share, and American Depositary Shares ("ADS"). Each ADS represents one Ordinary Share 06/17/2025 0001730463 Autolus Therapeutics plc 05280R100 THE MEDIAWORKS 191 WOOD LANE LONDON W3 7FP Rule 13d-1(c) MAK Capital Fund LP a D0 0.00 15408638.00 0.00 15408638.00 15408638.00 N 5.8 PN For item 6: Represents 15,408,638 shares of American Depositary Shares ("ADS"), each representing one ordinary share, par value $0.000042 per share. For item 11: The calculation is based upon 266,141,411 ordinary shares (including shares in the form of ADS), nominal value $0.000042 per share outstanding as of May 7, 2025, as reported by the Issuer on its Quarterly Report on Form 10-Q filed with the Securities and Exchange Commission on May 8, 2025. MAK Capital One L.L.C. a DE 0.00 15408638.00 0.00 15408638.00 15408638.00 N 5.8 IA For item 11: The calculation is based upon 266,141,411 ordinary shares (including shares in the form of ADS), nominal value $0.000042 per share outstanding as of May 7, 2025, as reported by the Issuer on its Quarterly Report on Form 10-Q filed with the Securities and Exchange Commission on May 8, 2025. Michael A. Kaufman a DE 0.00 15408638.00 0.00 15408638.00 15408638.00 N 5.8 IN For item 11: The calculation is based upon 266,141,411 ordinary shares (including shares in the form of ADS), nominal value $0.000042 per share outstanding as of May 7, 2025, as reported by the Issuer on its Quarterly Report on Form 10-Q filed with the Securities and Exchange Commission on May 8, 2025. Autolus Therapeutics plc The Mediaworks, 191 Wood Lane, London, W12 7FP, United Kingdom This Schedule 13G is being filed by i. MAK Capital Fund LP ("MAK Fund") ii. MAK Capital One L.L.C. ("MAK Capital"); and iii. Michael A. Kaufman ("Mr. Kaufman," and collectively with MAK Fund and MAK Capital, the "Reporting Persons") The principal business address of (i) MAK Fund is c/o Wakefield Quin, Victoria Place, 31 Victoria Street, Bermuda; and (ii) MAK Capital and Mr. Kaufman is 590 Madison Avenue, 31st Floor, New York, NY 10022. MAK Fund is a Bermuda limited partnership. MAK Capital is a Delaware limited liability company. Mr. Kaufman is a United States citizen. Y 15,408,638 5.8 The calculation is based upon 266,141,411 ordinary shares (including shares in the form of ADS), nominal value $0.000042 per share outstanding as of May 7, 2025, as reported by the Issuer on its Quarterly Report on Form 10-Q filed with the Securities and Exchange Commission on May 8, 2025. 0 15,408,638 0 15,408,638 Y Y Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. MAK Capital Fund LP /s/ Michael A. Kaufman 06/25/2025 MAK Capital One L.L.C. /s/ Michael A. Kaufman 06/25/2025 Michael A. Kaufman /s/ Michael A. Kaufman 06/25/2025