SCHEDULE 13G/A 0001725042 XXXXXXXX LIVE 8 Ordinary Shares, nominal value $0.000042 per share, and American Depositary Shares ("ADS"). Each ADS represents one Ordinary Share. 03/31/2025 0001730463 Autolus Therapeutics plc 05280R100 The Mediaworks, 191 Wood Lane London X0 W12 7FP Rule 13d-1(d) Syncona Portfolio Limited b Y7 0 28821053 0 28821053 28821053 N 10.8 CO Response to Items 6, 8 and 9 consist of 12,180,333 ordinary shares and 16,640,720 American Depositary Shares ("ADSs") held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value $0.000042 per share, of the Issuer. The ordinary shares and the ADSs are collectively referred to as the "Ordinary Shares". Response to Item 11 is based on 266,128,900 Ordinary Shares outstanding as reported in the Issuer's Form 10-K filed with the Securities and Exchange Commission (the "SEC") on March 20, 2025. Syncona Holdings Limited b Y7 0 28821053 0 28821053 28821053 N 10.8 CO Response to Items 6, 8 and 9 consist of 12,180,333 ordinary shares and 16,640,720 American Depositary Shares ("ADSs") held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value $0.000042 per share, of the Issuer. The ordinary shares and the ADSs are collectively referred to as the "Ordinary Shares". Response to Item 11 is based on 266,128,900 Ordinary Shares outstanding as reported in the Issuer's Form 10-K filed with the Securities and Exchange Commission (the "SEC") on March 20, 2025. Syncona Investment Management Limited b X0 0 28821053 0 28821053 28821053 N 10.8 CO Response to Items 6, 8 and 9 consist of 12,180,333 ordinary shares and 16,640,720 American Depositary Shares ("ADSs") held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value $0.000042 per share, of the Issuer. The ordinary shares and the ADSs are collectively referred to as the "Ordinary Shares". Response to Item 11 is based on 266,128,900 Ordinary Shares outstanding as reported in the Issuer's Form 10-K filed with the Securities and Exchange Commission (the "SEC") on March 20, 2025. Syncona Limited b Y7 0 28821053 0 28821053 28821053 N 10.8 CO Response to Items 6, 8 and 9 consist of 12,180,333 ordinary shares and 16,640,720 American Depositary Shares ("ADSs") held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value $0.000042 per share, of the Issuer. The ordinary shares and the ADSs are collectively referred to as the "Ordinary Shares". Response to Item 11 is based on 266,128,900 Ordinary Shares outstanding as reported in the Issuer's Form 10-K filed with the Securities and Exchange Commission (the "SEC") on March 20, 2025. Roel Bulthuis b P7 0 28821053 0 28821053 28821053 N 10.8 IN Response to Items 6, 8 and 9 consist of 12,180,333 ordinary shares and 16,640,720 American Depositary Shares ("ADSs") held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value $0.000042 per share, of the Issuer. The ordinary shares and the ADSs are collectively referred to as the "Ordinary Shares". Response to Item 11 is based on 266,128,900 Ordinary Shares outstanding as reported in the Issuer's Form 10-K filed with the Securities and Exchange Commission (the "SEC") on March 20, 2025. Christopher Hollowood b X0 0 28821053 0 28821053 28821053 N 10.8 IN Response to Items 6, 8 and 9 consist of 12,180,333 ordinary shares and 16,640,720 American Depositary Shares ("ADSs") held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value $0.000042 per share, of the Issuer. The ordinary shares and the ADSs are collectively referred to as the "Ordinary Shares". Response to Item 11 is based on 266,128,900 Ordinary Shares outstanding as reported in the Issuer's Form 10-K filed with the Securities and Exchange Commission (the "SEC") on March 20, 2025. Kenneth Galbraith b Z4 0 28821053 0 28821053 28821053 N 10.8 IN Response to Items 6, 8 and 9 consist of 12,180,333 ordinary shares and 16,640,720 American Depositary Shares ("ADSs") held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value $0.000042 per share, of the Issuer. The ordinary shares and the ADSs are collectively referred to as the "Ordinary Shares". Response to Item 11 is based on 266,128,900 Ordinary Shares outstanding as reported in the Issuer's Form 10-K filed with the Securities and Exchange Commission (the "SEC") on March 20, 2025. Autolus Therapeutics plc The Mediaworks, 191 Wood Lane, London W12 7FP, United Kingdom (i) Syncona Portfolio Limited; (ii) Syncona Holdings Limited; (iii)Syncona Investment Management Limited; (iv) Syncona Limited; (v) Roel Bulthuis; (vi) Christopher Hollowood; and (vii) Kenneth Galbraith The address of the principal business office of Syncona Portfolio Limited, Syncona Holdings Limited and Syncona Limited is Frances House, PO Box 273, Sir William Place, St. Peter Port, Guernsey, GY1 3RD, Channel Islands. The address of the principal business office of Syncona Investment Management Limited, Roel Bulthuis, Christopher Hollowood and Kenneth Galbraith is 2nd Floor, 8 Bloomsbury Street, London WC1B 3SR, United Kingdom. Syncona Portfolio Limited, Syncona Holdings Limited and Syncona Limited's citizenship is Guernsey. Syncona Investment Management Limited and Christopher Hollowood's citizenship is United Kingdom. Roel Bulthuis' citizenship is the Netherlands. Kenneth Galbraith's citizenship is Canada. Y 28,821,053 10.8 0 28,821,053 0 28,821,053 Y Y Y Y Y Y Syncona Portfolio Limited /s/ Nicholas Moss 05/07/2025 Syncona Holdings Limited /s/ Nicholas Moss 05/07/2025 Syncona Investment Management Limited /s/ Christopher Hollowood 05/07/2025 Syncona Limited /s/ Robert Hutchinson 05/07/2025 Roel Bulthuis /s/ Roel Bulthuis 05/07/2025 Christopher Hollowood /s/ Christopher Hollowood 05/07/2025 Kenneth Galbraith /s/ Kenneth Galbraith 05/07/2025