X0609 4 2026-06-16 0001980845 enGene Therapeutics Inc. ENGN 0001730229 Grossman William true C/O 4868 RUE LEVY, SUITE 220 SAINT-LAURENT A8 H4R 2P1 QUEBEC, CANADA true true Interim Chief Medical Officer false Stock Option (Right to Buy) 1.75 2026-06-16 4 A 0 31000 0 A 2036-06-16 Common Shares 31000 31000 D Stock Option (Right to Buy) 1.75 2026-06-16 4 A 0 200000 0 A 2036-06-16 Common Shares 200000 200000 D This option vests fully on the earlier of (i) the first anniversary of the vesting commencement date, which was June 9, 2026 or (ii) the registrant's 2027 annual meeting of shareholders. These options were granted to the reporting person on June 16, 2026 for his service as a non-executive director, consistent with annual grants made to other non-executive directors of the board. This option vests monthly in substantially equal amounts on the 12th day of each month for 12 consecutive months, subject to the reporting person's continued service as Interim Chief Medical Officer at the time of each vesting. These options were granted to the reporting person in connection with his appointment as the Interim Chief Medical Officer of the Company. /s/ Lee Giguere, as attorney-in-fact for the Reporting Person 2026-06-17