<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2020-10-20</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001724570</issuerCik>
        <issuerName>DATTO HOLDING CORP.</issuerName>
        <issuerTradingSymbol>MSP</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001665262</rptOwnerCik>
            <rptOwnerName>Vista Equity Partners Fund VI, L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O VISTA EQUITY PARTNERS</rptOwnerStreet1>
            <rptOwnerStreet2>4 EMBARCADERO CENTER, 20TH FL.</rptOwnerStreet2>
            <rptOwnerCity>SAN FRANCISCO</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94111</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001786024</rptOwnerCik>
            <rptOwnerName>Vista Equity Partners Fund VI GP, L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O VISTA EQUITY PARTNERS</rptOwnerStreet1>
            <rptOwnerStreet2>4 EMBARCADERO CENTER, 20TH FL.</rptOwnerStreet2>
            <rptOwnerCity>SAN FRANCISCO</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94111</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001786074</rptOwnerCik>
            <rptOwnerName>VEPF VI GP. Ltd.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O VISTA EQUITY PARTNERS</rptOwnerStreet1>
            <rptOwnerStreet2>4 EMBARCADERO CENTER, 20TH FL.</rptOwnerStreet2>
            <rptOwnerCity>SAN FRANCISCO</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94111</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001216497</rptOwnerCik>
            <rptOwnerName>SMITH ROBERT F</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O VISTA EQUITY PARTNERS</rptOwnerStreet1>
            <rptOwnerStreet2>401 CONGRESS DRIVE, SUITE 3100</rptOwnerStreet2>
            <rptOwnerCity>AUSTIN</rptOwnerCity>
            <rptOwnerState>TX</rptOwnerState>
            <rptOwnerZipCode>78701</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common stock, $0.001 par value</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>113753615</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See Footnote</value>
                    <footnoteId id="F1"/>
                    <footnoteId id="F2"/>
                    <footnoteId id="F3"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">Includes 13,941,912 shares held directly by Vista Foundation Fund II, L.P. (&quot;VFF II&quot;), 3,175,352 shares held directly by Vista Foundation Fund II-A, L.P. (&quot;VFF II-A&quot;), 846,946 shares held directly by VFF II FAF, L.P. (&quot;VFF II FAF&quot;), 208,600 shares held directly by Vista Foundation Fund II Executive, L.P. (&quot;VFF II Executive&quot;), 27,193 shares held directly by Vista Foundation Associates II, LLC (&quot;VF Associates II&quot;) and 95,553,611 shares held directly by Merritt VI Aggregator, LLC (&quot;Merritt VI,&quot; and collectively with VFF II, VFF II-A, VFF II FAF, VFF II Executive and VF Associates II, the &quot;Vista Funds&quot;).</footnote>
        <footnote id="F2">Vista Foundation Fund II GP, LLC (&quot;VFF II GP&quot;) is the general partner of each of VFF II, VFF II FAF and VFF II Executive. VEP Group, LLC (&quot;VEP Group&quot;) is the Senior Managing Member of VFF II GP and VF Associates II. VFF II GP (Cayman), L.P. (&quot;VFF II-A GP&quot;) is the general partner of VFF II-A. VFF II GP (Cayman), Ltd. (&quot;VFF II-A UGP&quot;) is the general partner of VFF II-A GP. Vista Equity Partners Fund VI, L.P. (&quot;VEPF VI&quot;) is the managing member of Merritt VI. Vista Equity Partners Fund VI GP, L.P. (&quot;Fund VI GP&quot;) is the sole general partner of VEPF VI. VEPF VI GP, Ltd. (&quot;Fund VI UGP&quot;) is the general partner of Fund VI GP. Robert F. Smith is the sole director and one of 11 members of each of VFF II-A UGP and Fund VI UGP. Robert F. Smith is the sole Managing Member of VEP Group.</footnote>
        <footnote id="F3">(Continued from footnote 2) Consequently, Mr. Smith, VFF II GP, VFF II-A GP, VFF II-A UGP, VEPF VI, Fund VI GP, Fund VI UGP and VEP Group may be deemed the beneficial owners of the shares held by the Vista Funds. Each of the Vista Funds, Mr. Smith, VFF II GP, VFF II-A GP, VFF II-A UGP, VEPF VI, Fund VI GP, Fund VI UGP and VEP Group expressly disclaim beneficial ownership of any shares not held directly, except to the extent of its or his pecuniary interest.</footnote>
    </footnotes>

    <remarks>Exhibit 24 - Power of Attorney. This report is filed as form 2 of 2 to report related transactions for the following filers: Vista Foundation Fund II, L.P., Vista Foundation Fund II-A, L.P., VFF II FAF, L.P., Vista Foundation Fund II Executive, L.P., Vista Foundation Associates II, LLC, Merritt VI Aggregator, LLC, Vista Foundation Fund II GP, LLC, VEP Group, LLC, VFF II GP (Cayman), L.P., VFF II GP (Cayman), Ltd., Vista Equity Partners Fund VI, L.P., Vista Equity Partners Fund VI GP, L.P., VEPF VI GP, Ltd. and Robert F. Smith.</remarks>

    <ownerSignature>
        <signatureName>/s/ Robert F. Smith, sole director and member of the General Partner of the General Partner of Vista Equity Partners Fund VI, L.P.</signatureName>
        <signatureDate>2020-10-20</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Robert F. Smith, sole director and member of the General Partner of Vista Equity Partners Fund VI GP, L.P.</signatureName>
        <signatureDate>2020-10-20</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Robert F. Smith, sole director and member of VEPF VIGP, Ltd.</signatureName>
        <signatureDate>2020-10-20</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Robert F. Smith</signatureName>
        <signatureDate>2020-10-20</signatureDate>
    </ownerSignature>
</ownershipDocument>
