X0206 3 2020-06-25 0 0001722271 Akouos, Inc. AKUS 0001664281 PARMAR KUSH 501 SECOND STREET, SUITE 350 SAN FRANCISCO CA 94107 1 0 1 0 Series Seed Preferred Stock Common Stock 513757 I See Footnotes Series A Preferred Stock Common Stock 1997946 I See Footnotes Series B Preferred Stock Common Stock 1799964 I See Footnotes The Series Seed Preferred Stock is convertible into Common Stock on a 21.073-for-1 basis into the number of shares of Common Stock as shown in Column 3 at the holder's election or upon closing of the initial public offering of the Issuer's common stock without payment of further consideration. The shares have no expiration date. Consists of 513,757 shares held by 5AM Ventures V, L.P. ("Ventures V"). 5AM Partners V, LLC is the general partner of Ventures V and may be deemed to have sole investment and voting power over the shares held by Ventures V. The Reporting Person is a Managing Member of 5AM Partners V, LLC, and may be deemed to have shared voting and dispositive power over the shares held by Ventures V. 5AM Opportunities I (GP), LLC is the general partner of 5AM Opportunities I, L.P. ("Opportunities I") and may be deemed to have sole investment and voting power over the shares held by Opportunities I. The Reporting Person is a Managing Member of 5AM Opportunities I (GP), LLC, and may be deemed to have shared voting and dispositive power over the shares held by Opportunities I. Therefore, the Reporting Person may be deemed to beneficially own the shares held by Ventures V and Opportunities I. The Reporting Person disclaims beneficial ownership over the shares held by Ventures V and Opportunities I except to the extent of his pecuniary interest therein. The Series A Preferred Stock is convertible into Common Stock on a 21.073-for-1 basis into the number of shares of Common Stock as shown in Column 3 at the holder's election or upon closing of the initial public offering of the Issuer's common stock without payment of further consideration. The shares have no expiration date. Consists of 1,997,946 shares held by Ventures V. The Series B Preferred Stock is convertible into Common Stock on a 21.073-for-1 basis into the number of shares of Common Stock as shown in Column 3 at the holder's election or upon closing of the initial public offering of the Issuer's common stock without payment of further consideration. The shares have no expiration date. Consists of 999,980 shares held by Ventures V and 799,984 shares held by Opportunities I. Exhibit Index: 24.1 Power of Attorney /s/ Karoline K. Shair, as attorney-in-fact for Kush Parmar 2020-06-25