Exhibit 23.1

Consent of Independent Registered Public Accounting Firm

We consent to the incorporation by reference in the following Registration Statements:

(1) Form S-8 No. 333-240087 pertaining to the Jamf Holding Corp. Omnibus Incentive Plan and the Amended and Restated Jamf Holding Corp. 2017 Stock Option Plan of Jamf Holding Corp.,

(2) Form S-8 No. 333-264487 pertaining to the Employee Stock Purchase Plan of Jamf Holding Corp., and

(3) Form S-3 No. 333-265821 of Jamf Holding Corp. pertaining to the registration of common shares

of our reports dated February 27, 2024, with respect to the consolidated financial statements of Jamf Holding Corp. and the effectiveness of internal control over financial reporting of Jamf Holding Corp. included in this Annual Report (Form 10-K) for the year ended December 31, 2023.

/s/ Ernst & Young LLP

Minneapolis, Minnesota

February 27, 2024