<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2017-12-13</periodOfReport>

    <notSubjectToSection16>1</notSubjectToSection16>

    <issuer>
        <issuerCik>0001589149</issuerCik>
        <issuerName>MassRoots, Inc.</issuerName>
        <issuerTradingSymbol>MSRT</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001720321</rptOwnerCik>
            <rptOwnerName>Kveton Scott C</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>1624 MARKET STREET</rptOwnerStreet1>
            <rptOwnerStreet2>SUITE 201</rptOwnerStreet2>
            <rptOwnerCity>DENVER</rptOwnerCity>
            <rptOwnerState>CO</rptOwnerState>
            <rptOwnerZipCode>80202</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>Chief Executive Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>2826877</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">48,106 shares of Common Stock included herein are held by Mr. Kveton and Sarah Tuthill-Kveton, as joint tenants with right of survivorship. A total of 1,550,000 shares issued pursuant to an employment agreement in June 2017, originally vesting over a term of 24 months, fully vested on December 13, 2017, as per the terms of a Separation Agreement of the same date, concurrent with which, Mr. Kveton also resigned as an officer of the Company.</footnote>
    </footnotes>

    <remarks>On December 13, 2017 the Reporting Owner resigned as Chief Executive Officer of the Reporting Issuer.</remarks>

    <ownerSignature>
        <signatureName>/s/Scott C. Kveton</signatureName>
        <signatureDate>2018-03-12</signatureDate>
    </ownerSignature>
</ownershipDocument>
