144 0001114357 XXXXXXXX LIVE 0001717115 TEMPUS AI, INC. 001-42130 600 WEST CHICAGO AVENUE, SUITE 510 CHICAGO IL 60654 800-976-5448 Eric Paul Lefkofsky Officer Director 10% Stockholder Class A Common Stock Merrill Lynch, Pierce, Fenner & Smith Incorporated

249 Royal Palm Way, Ste 200 Palm Beach FL 33480

400000 28800000.00 168072456 06/17/2025 Nasdaq Class A Common Stock Morgan Stanley Smith Barney LLC

1 New York Plaza, 8th Floor New York NY 10004

100000 7200000.00 168072456 06/17/2025 Nasdaq Class A Common Stock 04/20/2017 Direct Investment Issuer N 400000 04/20/2017 Cash Class A Common Stock 09/14/2017 Direct Investment Issuer N 100000 09/14/2017 Cash N Eric P. Lefkofsky

c/o Tempus AI, Inc. 600 West Chicago Avenue, Suite 510 Chicago IL 60654

Class A Common Stock 05/20/2025 16752 1067772.48 This form relates to sales by Blue Media, LLC (400,000 shares) and Gray Media, LLC (100,000 shares), of which Mr. Lefkofsky is the sole manager. The foregoing sales are being made by multiple brokers pursuant to a single trading plan adopted in accordance with Rule 10b5-1 under the Exchange Act. 06/17/2025 06/14/2024 /s/ Eric P. Lefkofsky