144 0001754464 XXXXXXXX LIVE 0001717115 TEMPUS AI, INC. 001-42130 600 WEST CHICAGO AVENUE, SUITE 510 CHICAGO IL 60654 800-976-5448 Ryan M. Bartolucci Officer Class A Common Stock Morgan Stanley Smith Barney LLC Executive Financial Services

1 New York Plaza, 8th Floor New York NY 10004

4254 271149.96 168072456 05/20/2025 NASDAQ Class A Common Stock 07/14/2021 Restricted Stock Units Issuer N 782 07/14/2021 N/A Class A Common Stock 04/27/2022 Restricted Stock Units Issuer N 336 04/27/2022 N/A Class A Common Stock 04/18/2023 Restricted Stock Units Issuer N 336 04/18/2023 N/A Class A Common Stock 05/02/2024 Restricted Stock Units Issuer N 1490 05/02/2024 N/A Class A Common Stock 02/24/2025 Restricted Stock Units Issuer N 1310 02/24/2025 N/A Y The sales reported on this Form 144 are mandated by the Issuer's election under its equity incentive plan to require the satisfaction of a tax withholding obligation to be funded by sell to cover transactions and do not represent discretionary trades by the reporting person. 05/21/2025 /s/ Ryan M Bartolucci