<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
	<headerData>
        <submissionType>144</submissionType>
                <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001114357</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
        <liveTestFlag>LIVE</liveTestFlag>




                </filerInfo>
    </headerData>

     <formData>
        <issuerInfo>
            <issuerCik>0001717115</issuerCik>
			<issuerName>TEMPUS AI, INC.</issuerName>


			    <secFileNumber>001-42130</secFileNumber>


			<issuerAddress>
				<com:street1>600 WEST CHICAGO AVENUE, SUITE 510</com:street1>
                				<com:city>CHICAGO</com:city>
				<com:stateOrCountry>IL</com:stateOrCountry>
				<com:zipCode>60654</com:zipCode>
			</issuerAddress>

			<issuerContactPhone>800-976-5448</issuerContactPhone>
			<nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Eric Paul Lefkofsky</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>

			<relationshipsToIssuer>

				    <relationshipToIssuer>Officer</relationshipToIssuer>
                                				    <relationshipToIssuer>Director</relationshipToIssuer>
                                				    <relationshipToIssuer>10% Stockholder</relationshipToIssuer>

			</relationshipsToIssuer>
		</issuerInfo>

				          <securitiesInformation>
			<securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
			<brokerOrMarketmakerDetails>
				<name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
				<address>
				<com:street1>1 New York Plaza, 8th Floor</com:street1>
                				<com:city>New York</com:city>
				<com:stateOrCountry>NY</com:stateOrCountry>
				<com:zipCode>10004</com:zipCode>
				</address>
			</brokerOrMarketmakerDetails>
			<noOfUnitsSold>16752</noOfUnitsSold>
			<aggregateMarketValue>1067772.48</aggregateMarketValue>
			<noOfUnitsOutstanding>168072456</noOfUnitsOutstanding>
			<approxSaleDate>05/20/2025</approxSaleDate>
			<securitiesExchangeName>NASDAQ</securitiesExchangeName>
		</securitiesInformation>

    		       <securitiesToBeSold>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <acquiredDate>06/13/2024</acquiredDate>
         <natureOfAcquisitionTransaction>Restricted Stock Units</natureOfAcquisitionTransaction>
                  <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
                  <isGiftTransaction>N</isGiftTransaction>
		          <amountOfSecuritiesAcquired>16752</amountOfSecuritiesAcquired>
                  <paymentDate>06/13/2024</paymentDate>
                  <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>

    <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>


     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Gray Media, LLC</name>
            <address>
               <com:street1>600 WEST CHICAGO AVENUE, Suite 510</com:street1>
                              <com:city>CHICAGO</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>03/06/2025</saleDate>
         <amountOfSecuritiesSold>50000</amountOfSecuritiesSold>
         <grossProceeds>2485384.77</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Blue Media, LLC</name>
            <address>
               <com:street1>600 WEST CHICAGO AVENUE, Suite 510</com:street1>
                              <com:city>CHICAGO</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>03/06/2025</saleDate>
         <amountOfSecuritiesSold>200000</amountOfSecuritiesSold>
         <grossProceeds>9972430.75</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Gray Media, LLC</name>
            <address>
               <com:street1>600 WEST CHICAGO AVENUE, Suite 510</com:street1>
                              <com:city>CHICAGO</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>03/05/2025</saleDate>
         <amountOfSecuritiesSold>100000</amountOfSecuritiesSold>
         <grossProceeds>5173986.28</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Blue Media, LLC</name>
            <address>
               <com:street1> 600 WEST CHICAGO AVENUE, Suite 510</com:street1>
                              <com:city>CHICAGO</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>03/05/2025</saleDate>
         <amountOfSecuritiesSold>462354</amountOfSecuritiesSold>
         <grossProceeds>23467279.92</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Lightbank Global LLC</name>
            <address>
               <com:street1>600 WEST CHICAGO AVENUE, Suite 510</com:street1>
                              <com:city>CHICAGO</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/27/2025</saleDate>
         <amountOfSecuritiesSold>1397589</amountOfSecuritiesSold>
         <grossProceeds>82108353.75</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Innovation Group Investors, L.P. - Series 1B</name>
            <address>
               <com:street1>600 WEST CHICAGO AVENUE, Suite 510</com:street1>
                              <com:city>CHICAGO</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/27/2025</saleDate>
         <amountOfSecuritiesSold>145966</amountOfSecuritiesSold>
         <grossProceeds>8575502.5</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Lightbank Investments 1B, LLC</name>
            <address>
               <com:street1>600 WEST CHICAGO AVENUE, Suite 510</com:street1>
                              <com:city>CHICAGO</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/27/2025</saleDate>
         <amountOfSecuritiesSold>115751</amountOfSecuritiesSold>
         <grossProceeds>6800371.25</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Innovation Group Investors, L.P. - 2011 Series</name>
            <address>
               <com:street1>600 WEST CHICAGO AVENUE, Suite 510</com:street1>
                              <com:city>CHICAGO</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/27/2025</saleDate>
         <amountOfSecuritiesSold>1580928</amountOfSecuritiesSold>
         <grossProceeds>92879520</grossProceeds>
      </securitiesSoldInPast3Months>

    		<remarks>The sales reported on this Form 144 are mandated by the Issuer's election under its equity incentive plan to require the satisfaction of a tax withholding obligation to be funded by sell to cover transactions and do not represent discretionary trades by the reporting person.</remarks>

        <noticeSignature>
            <noticeDate>05/21/2025</noticeDate>
                        <signature> /s/ Eric P. Lefkofsky</signature>
        </noticeSignature>
	</formData>
    </edgarSubmission>
