<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
	<headerData>
        <submissionType>144</submissionType>
                <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001114357</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
        <liveTestFlag>LIVE</liveTestFlag>




                </filerInfo>
    </headerData>

     <formData>
        <issuerInfo>
            <issuerCik>0001717115</issuerCik>
			<issuerName>TEMPUS AI, INC.</issuerName>


			    <secFileNumber>001-42130</secFileNumber>


			<issuerAddress>
				<com:street1>600 WEST CHICAGO AVENUE, SUITE 510</com:street1>
                				<com:city>CHICAGO</com:city>
				<com:stateOrCountry>IL</com:stateOrCountry>
				<com:zipCode>60654</com:zipCode>
			</issuerAddress>

			<issuerContactPhone>800-976-5448</issuerContactPhone>
			<nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Eric P. Lefkofsky</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>

			<relationshipsToIssuer>

				    <relationshipToIssuer>Officer</relationshipToIssuer>
                                				    <relationshipToIssuer>Director</relationshipToIssuer>
                                				    <relationshipToIssuer>10% Stockholder</relationshipToIssuer>

			</relationshipsToIssuer>
		</issuerInfo>

				          <securitiesInformation>
			<securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
			<brokerOrMarketmakerDetails>
				<name>Merrill Lynch, Pierce, Fenner &amp; Smith Incorporated</name>
				<address>
				<com:street1>249 Royal Palm Way, Ste 200</com:street1>
                				<com:city>Palm Beach</com:city>
				<com:stateOrCountry>FL</com:stateOrCountry>
				<com:zipCode>33480</com:zipCode>
				</address>
			</brokerOrMarketmakerDetails>
			<noOfUnitsSold>662345</noOfUnitsSold>
			<aggregateMarketValue>34925452</aggregateMarketValue>
			<noOfUnitsOutstanding>167168911</noOfUnitsOutstanding>
			<approxSaleDate>03/05/2025</approxSaleDate>
			<securitiesExchangeName>Nasdaq</securitiesExchangeName>
		</securitiesInformation>
                     <securitiesInformation>
			<securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
			<brokerOrMarketmakerDetails>
				<name>Morgan Stanley Smith Barney LLC</name>
				<address>
				<com:street1>1 New York Plaza, 8th Floor</com:street1>
                				<com:city>New York</com:city>
				<com:stateOrCountry>NY</com:stateOrCountry>
				<com:zipCode>10004</com:zipCode>
				</address>
			</brokerOrMarketmakerDetails>
			<noOfUnitsSold>150000</noOfUnitsSold>
			<aggregateMarketValue>7909500</aggregateMarketValue>
			<noOfUnitsOutstanding>167168911</noOfUnitsOutstanding>
			<approxSaleDate>03/05/2025</approxSaleDate>
			<securitiesExchangeName>Nasdaq</securitiesExchangeName>
		</securitiesInformation>

    		       <securitiesToBeSold>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <acquiredDate>04/20/2017</acquiredDate>
         <natureOfAcquisitionTransaction>Direct Investment</natureOfAcquisitionTransaction>
                  <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
                  <isGiftTransaction>N</isGiftTransaction>
		          <amountOfSecuritiesAcquired>662345</amountOfSecuritiesAcquired>
                  <paymentDate>04/20/2017</paymentDate>
                  <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
             <securitiesToBeSold>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <acquiredDate>09/14/2017</acquiredDate>
         <natureOfAcquisitionTransaction>Direct Investment</natureOfAcquisitionTransaction>
                  <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
                  <isGiftTransaction>N</isGiftTransaction>
		          <amountOfSecuritiesAcquired>150000</amountOfSecuritiesAcquired>
                  <paymentDate>09/14/2017</paymentDate>
                  <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>

    <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>


     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Lightbank Global LLC</name>
            <address>
               <com:street1>600 W Chicago Avenue</com:street1>
                              <com:street2>Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/27/2025</saleDate>
         <amountOfSecuritiesSold>1397589</amountOfSecuritiesSold>
         <grossProceeds>82108353.75</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Innovation Group Investors, L.P. - Series 1B</name>
            <address>
               <com:street1>600 W Chicago Avenue</com:street1>
                              <com:street2>Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/27/2025</saleDate>
         <amountOfSecuritiesSold>145966</amountOfSecuritiesSold>
         <grossProceeds>8575502.5</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Lightbank Investments 1B, LLC</name>
            <address>
               <com:street1>600 W Chicago Avenue</com:street1>
                              <com:street2>Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/27/2025</saleDate>
         <amountOfSecuritiesSold>115751</amountOfSecuritiesSold>
         <grossProceeds>6800371.25</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Innovation Group Investors, L.P. - 2011 Series</name>
            <address>
               <com:street1>600 W Chicago Avenue</com:street1>
                              <com:street2>Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/27/2025</saleDate>
         <amountOfSecuritiesSold>1580928</amountOfSecuritiesSold>
         <grossProceeds>92879520</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/20/2025</saleDate>
         <amountOfSecuritiesSold>69231</amountOfSecuritiesSold>
         <grossProceeds>5129762.85</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/19/2025</saleDate>
         <amountOfSecuritiesSold>84640</amountOfSecuritiesSold>
         <grossProceeds>6844874.78</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/14/2025</saleDate>
         <amountOfSecuritiesSold>80003</amountOfSecuritiesSold>
         <grossProceeds>6862444.28</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/13/2025</saleDate>
         <amountOfSecuritiesSold>78594</amountOfSecuritiesSold>
         <grossProceeds>6170834.61</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/12/2025</saleDate>
         <amountOfSecuritiesSold>84948</amountOfSecuritiesSold>
         <grossProceeds>5947800.43</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/07/2025</saleDate>
         <amountOfSecuritiesSold>80388</amountOfSecuritiesSold>
         <grossProceeds>5428310.15</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/06/2025</saleDate>
         <amountOfSecuritiesSold>85109</amountOfSecuritiesSold>
         <grossProceeds>5778223.42</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>02/05/2025</saleDate>
         <amountOfSecuritiesSold>74485</amountOfSecuritiesSold>
         <grossProceeds>5030008.45</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>01/31/2025</saleDate>
         <amountOfSecuritiesSold>82056</amountOfSecuritiesSold>
         <grossProceeds>4776783.87</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>01/30/2025</saleDate>
         <amountOfSecuritiesSold>77626</amountOfSecuritiesSold>
         <grossProceeds>4240573.32</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>01/29/2025</saleDate>
         <amountOfSecuritiesSold>79118</amountOfSecuritiesSold>
         <grossProceeds>4261440.36</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>01/24/2025</saleDate>
         <amountOfSecuritiesSold>85788</amountOfSecuritiesSold>
         <grossProceeds>4580521.6</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>01/23/2025</saleDate>
         <amountOfSecuritiesSold>645068</amountOfSecuritiesSold>
         <grossProceeds>33710610.15</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Eric P. Lefkofsky</name>
            <address>
               <com:street1>c/o Tempus AI, Inc.</com:street1>
                              <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>01/22/2025</saleDate>
         <amountOfSecuritiesSold>476209</amountOfSecuritiesSold>
         <grossProceeds>23733981.2</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Gray Media, LLC</name>
            <address>
               <com:street1>600 W Chicago Avenue</com:street1>
                              <com:street2>Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>12/18/2024</saleDate>
         <amountOfSecuritiesSold>50000</amountOfSecuritiesSold>
         <grossProceeds>1879581.97</grossProceeds>
      </securitiesSoldInPast3Months>

     <securitiesSoldInPast3Months>
         <sellerDetails>
            <name>Blue Media, LLC</name>
            <address>
               <com:street1>600 W Chicago Avenue</com:street1>
                              <com:street2>Suite 510</com:street2>
                              <com:city>Chicago</com:city>
               <com:stateOrCountry>IL</com:stateOrCountry>
               <com:zipCode>60654</com:zipCode>
            </address>
         </sellerDetails>
         <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
         <saleDate>12/18/2024</saleDate>
         <amountOfSecuritiesSold>137655</amountOfSecuritiesSold>
         <grossProceeds>5166041.39</grossProceeds>
      </securitiesSoldInPast3Months>

    		<remarks>This form relates to sales by Blue Media, LLC (662,345 shares) and Gray Media, LLC (150,000 shares), of which Mr. Lefkofsky is the sole manager. The foregoing sales are being made by multiple brokers pursuant to a single trading plan adopted in accordance with Rule 10b5-1 under the Exchange Act.</remarks>

        <noticeSignature>
            <noticeDate>03/05/2025</noticeDate>
                        <planAdoptionDates>
		                                     <planAdoptionDate>06/14/2024</planAdoptionDate>

            </planAdoptionDates>
                        <signature> /s/ Eric P. Lefkofsky</signature>
        </noticeSignature>
	</formData>
    </edgarSubmission>
