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<headerData>
    <submissionType>SCHEDULE 13G/A</submissionType>
    <previousAccessionNumber>0001088875-24-000121</previousAccessionNumber>
    <filerInfo>
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          <cik>0001088875</cik>
          <ccc>XXXXXXXX</ccc>
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      <liveTestFlag>LIVE</liveTestFlag>



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  </headerData>
  <formData>
    <coverPageHeader>
      <amendmentNo>4</amendmentNo>
      <securitiesClassTitle>Common Stock</securitiesClassTitle>
      <eventDateRequiresFilingThisStatement>03/31/2026</eventDateRequiresFilingThisStatement>
      <issuerInfo>
        <issuerCik>0001717115</issuerCik>
        <issuerName>Tempus AI, Inc.</issuerName>
        <issuerCusips>
          <issuerCusipNumber>88023B103</issuerCusipNumber>
        </issuerCusips>
        <issuerPrincipalExecutiveOfficeAddress>
          <com:street1>600 WEST CHICAGO AVENUE</com:street1>
          <com:street2>600 WEST CHICAGO AVENUE</com:street2>
          <com:city>CHICAGO</com:city>
          <com:stateOrCountry>IL</com:stateOrCountry>
          <com:zipCode>60654</com:zipCode>
        </issuerPrincipalExecutiveOfficeAddress>
      </issuerInfo>
      <designateRulesPursuantThisScheduleFiled>
        <designateRulePursuantThisScheduleFiled>Rule 13d-1(b)</designateRulePursuantThisScheduleFiled>
      </designateRulesPursuantThisScheduleFiled>
    </coverPageHeader>
    <coverPageHeaderReportingPersonDetails>

      <reportingPersonName>BAILLIE GIFFORD &amp; CO</reportingPersonName>
      <reportingPersonBeneficiallyOwnedNumberOfShares>
        <soleVotingPower>8615766.00</soleVotingPower>
        <sharedVotingPower>0.00</sharedVotingPower>
        <soleDispositivePower>8649356.00</soleDispositivePower>
        <sharedDispositivePower>0.00</sharedDispositivePower>
      </reportingPersonBeneficiallyOwnedNumberOfShares>
      <reportingPersonBeneficiallyOwnedAggregateNumberOfShares>8649356.00</reportingPersonBeneficiallyOwnedAggregateNumberOfShares>
      <classPercent>5.0</classPercent>
      <typeOfReportingPerson>IA</typeOfReportingPerson>
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    <items>
      <item1>
        <issuerName>Tempus AI, Inc.</issuerName>
        <issuerPrincipalExecutiveOfficeAddress>600 WEST CHICAGO AVENUE, 600 WEST CHICAGO AVENUE, CHICAGO, ILLINOIS, 60654</issuerPrincipalExecutiveOfficeAddress>
      </item1>
      <item2>
        <filingPersonName>Baillie Gifford &amp; Co</filingPersonName>
        <principalBusinessOfficeOrResidenceAddress>Calton Square,
1 Greenside Row,
Edinburgh,
EH1 4AN
UK</principalBusinessOfficeOrResidenceAddress>
        <citizenship>Scotland</citizenship>
      </item2>
      <item3>
        <notApplicableFlag>N</notApplicableFlag>
        <typeOfPersonFiling>FI</typeOfPersonFiling>
        <otherTypeOfPersonFiling>Investment Adviser</otherTypeOfPersonFiling>
      </item3>
      <item4>
        <amountBeneficiallyOwned>8,649,356</amountBeneficiallyOwned>
        <classPercent>4.98</classPercent>
        <numberOfSharesPersonHas>
          <solePowerOrDirectToVote>8,615,766</solePowerOrDirectToVote>
          <sharedPowerOrDirectToVote>0</sharedPowerOrDirectToVote>
          <solePowerOrDirectToDispose>8,649,356</solePowerOrDirectToDispose>
          <sharedPowerOrDirectToDispose>0</sharedPowerOrDirectToDispose>
        </numberOfSharesPersonHas>
      </item4>
      <item5>
        <classOwnership5PercentOrLess>Y</classOwnership5PercentOrLess>
      </item5>
      <item6>
        <notApplicableFlag>Y</notApplicableFlag>
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      <item7>
        <notApplicableFlag>Y</notApplicableFlag>
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      <item8>
        <notApplicableFlag>Y</notApplicableFlag>
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      <item9>
        <notApplicableFlag>Y</notApplicableFlag>
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      <item10>
        <notApplicableFlag>N</notApplicableFlag>
        <certifications>By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were acquired and are held in the ordinary course of business and were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ? 240.14a-11.

By signing below I certify that, to the best of my knowledge and belief, the foreign regulatory scheme applicable to Investment Adviser is substantially comparable to the regulatory scheme applicable to the functionally equivalent U.S. institution(s). I also undertake to furnish to the Commission staff, upon request, information that would otherwise be disclosed in a Schedule 13D.

</certifications>
      </item10>
    </items>
    <signatureInformation>
      <reportingPersonName>BAILLIE GIFFORD &amp; CO</reportingPersonName>
      <signatureDetails>
        <signature>Grant Meikle</signature>
        <title>Regulatory Reporting Manager</title>
        <date>05/07/2026</date>
      </signatureDetails>
    </signatureInformation>
  </formData>
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