X0306 4 2023-01-03 0 0001714899 Denali Therapeutics Inc. DNLI 0001724605 Ho Carole C/O DENALI THERAPEUTICS INC. 161 OYSTER POINT BLVD. SOUTH SAN FRANCISCO CA 94080 0 1 0 0 Chief Medical Officer Common Stock 2023-01-03 4 A 0 41120 0.00 A 138967 D Common Stock 2023-01-04 4 S 0 2913 28.69 D 175500 I See footnote Common Stock 25000 I See footnote Stock Option (right to buy) 27.63 2023-01-03 4 A 0 123360 0.00 A 2033-01-02 Common Stock 123360 123360 D Each share is represented by a Restricted Stock Unit ("RSU") and a contingent right to receive one share of common stock of the Issuer. 25% of the RSUs shall vest on January 3, 2024 (the "Vesting Commencement Date") and an additional 25% of the RSUs shall vest on each annual anniversary of the Vesting Commencement Date thereafter, subject to the Reporting Person remaining a service provider of the Issuer through each such date. Includes 138,557 RSUs. Reflects the issuance of shares to the Rohatgi-Ho Family 2009 Revocable Trust in connection with the vesting of 7,750 RSUs held by the Reporting Person. Shares sold to satisfy the tax obligations by the Reporting Person in connection with the settlement of previously vested RSUs. The sale price reported in column 4 of Table I represents the weighted average sale price of the shares ranging from $28.48 to $28.97 per share. Upon request by the Commission staff, the Issuer, or a security holder of the Issuer, the Reporting Person will provide full information regarding the number of shares sold at each separate price. The shares are held of record by the Rohatgi-Ho Family 2009 Revocable Trust, for which Reporting Person serves as trustee. The shares are held of record by the Rohatgi-Ho Irrevocable GST Trust for the benefit of the Reporting Person's children. 25% of the shares subject to the option shall vest on January 3, 2024, and 1/48 of the shares subject to the option shall vest each month thereafter, subject to the Reporting Person remaining a service provider of the Issuer through each such date. /s/ Tyler Nielsen, by power of attorney 2023-01-05