X0407 4 2023-06-01 0 0001711933 Akoya Biosciences, Inc. AKYA 0001271857 SCHNETTLER THOMAS P 100 CAMPUS DRIVE, 6TH FLOOR MARLBOROUGH MA 01762 1 0 0 0 0 Common Stock 0 I See footnote Stock Option (Right to Buy) 5.70 2023-06-01 4 A 0 56322 0.00 A 2033-06-01 Common Stock 56322 56322 D The Reporting Person is no longer reporting indirect ownership of shares held by Piper Sandler Merchant Banking Fund II, L.P. as a group of three or more individuals have voting and dispositive control over such shares, with each individual having one vote, and the approval of a majority of such individuals is required to approve an action; therefore, under the so-called "rule of three," no one individual is deemed to have or share beneficial ownership of such shares. Subject to the reporting person's continuous service through the vesting date, the options shall vest in full on the earlier of (a) June 1, 2024, or (b) the date of the next annual meeting of Akoya Biosciences, Inc. occurring after the date of grant. /s/ Thomas P. Schnettler, by Brian McKelligon, as Attorney-in-Fact 2023-06-05