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<ownershipDocument>

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    <periodOfReport>2025-07-08</periodOfReport>

    <notSubjectToSection16>1</notSubjectToSection16>

    <issuer>
        <issuerCik>0001711933</issuerCik>
        <issuerName>Akoya Biosciences, Inc.</issuerName>
        <issuerTradingSymbol>AKYA</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001857447</rptOwnerCik>
            <rptOwnerName>McKelligon Brian</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O AKOYA BIOSCIENCES, INC.</rptOwnerStreet1>
            <rptOwnerStreet2>100 CAMPUS DRIVE, 6TH FLOOR</rptOwnerStreet2>
            <rptOwnerCity>MARLBOROUGH</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>01752</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
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            <isDirector>1</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>President and CEO</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

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    <derivativeTable>
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            <securityTitle>
                <value>Employee Stock Option (Right to Buy)</value>
            </securityTitle>
            <conversionOrExercisePrice>
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            <transactionDate>
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            <exerciseDate>
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            <expirationDate>
                <value>2027-11-09</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
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                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>393338</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
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        <derivativeTransaction>
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            <transactionDate>
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                <transactionFormType>4</transactionFormType>
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                <transactionPricePerShare>
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            <exerciseDate>
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            <expirationDate>
                <value>2029-05-02</value>
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            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>331090</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
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        <derivativeTransaction>
            <securityTitle>
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                <value>16.12</value>
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            <transactionDate>
                <value>2025-07-08</value>
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                <transactionPricePerShare>
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                    <value>D</value>
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            <exerciseDate>
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            <expirationDate>
                <value>2031-03-24</value>
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            <underlyingSecurity>
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        <derivativeTransaction>
            <securityTitle>
                <value>Employee Stock Option (Right to Buy)</value>
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                <value>2025-07-08</value>
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                <equitySwapInvolved>0</equitySwapInvolved>
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                <transactionPricePerShare>
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                    <value>D</value>
                </transactionAcquiredDisposedCode>
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            <exerciseDate>
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            <expirationDate>
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            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
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                <underlyingSecurityShares>
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        <derivativeTransaction>
            <securityTitle>
                <value>Employee Stock Option (Right to Buy)</value>
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            <transactionDate>
                <value>2025-07-08</value>
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            <exerciseDate>
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            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
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                <directOrIndirectOwnership>
                    <value>D</value>
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        <derivativeTransaction>
            <securityTitle>
                <value>Employee Stock Option (Right to Buy)</value>
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                <transactionShares>
                    <value>175000</value>
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                    <value>D</value>
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                <value>2034-02-22</value>
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            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>175000</value>
                </underlyingSecurityShares>
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                <sharesOwnedFollowingTransaction>
                    <value>0</value>
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    <footnotes>
        <footnote id="F1">Cashless exercise of options pursuant to that certain Amended and Restated Agreement and Plan of Merger dated as of April 28, 2025, as amended (the &quot;Merger Agreement&quot;), by and among Quanterix Corporation, a Delaware corporation (&quot;Quanterix&quot;), Wellfleet Merger Sub, Inc., a Delaware corporation and wholly owned subsidiary of Quanterix (&quot;Merger Sub&quot;), and the Issuer. On July 8, 2025 (the &quot;Closing Date&quot;), Merger Sub merged with and into Issuer (the &quot;Merger&quot;), with Issuer surviving the Merger as a wholly owned subsidiary of Quanterix.</footnote>
        <footnote id="F2">Pursuant to the Merger Agreement, as of the Closing Date, each outstanding option to purchase Issuer common stock was accelerated and each option with a per share exercise price less than the value of the Per Share Merger Consideration (as defined below) was automatically cashless exercised.</footnote>
        <footnote id="F3">Disposition of shares pursuant to Merger Agreement.</footnote>
        <footnote id="F4">In connection with the terms of the Merger Agreement, each share of common stock of Issuer outstanding on the Closing Date was converted into the right to receive (a) 0.1461 of a share of common stock of Quanterix (the &quot;Per Share Stock Consideration&quot;) and (b) $0.38 in cash, without interest (the &quot;Per Share Cash Consideration&quot; and together with the Per Share Stock Consideration, the &quot;Per Share Merger Consideration&quot;). Each of the Per Share Stock Consideration and the Per Share Cash Consideration may be adjusted pursuant to the terms of the Merger Agreement.</footnote>
        <footnote id="F5">Disposition of options pursuant to the Merger Agreement.</footnote>
        <footnote id="F6">Pursuant to the Merger Agreement, as of the Closing Date, each outstanding option to purchase Issuer common stock was accelerated and each option with a per share exercise price equal to or greater than the Per Share Merger Consideration was automatically terminated and cancelled for no consideration.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Brian McKelligon</signatureName>
        <signatureDate>2025-07-10</signatureDate>
    </ownerSignature>
</ownershipDocument>
