Exhibit 23.1

Consent of Independent Registered Public Accounting Firm

We consent to the incorporation by reference in the registration statements (Nos. 333-273303 and 333-271167) on Form S-3ASR and (Nos. 333-269539, 333-262825, 333-252351, and 333-220589) on Form S-8 of our reports dated February 26, 2024, with respect to the consolidated financial statements of Krystal Biotech, Inc. and the effectiveness of internal control over financial reporting.

/s/ KPMG LLP

Pittsburgh, Pennsylvania

February 26, 2024