X0306 4 2022-06-07 0 0001636282 Aeglea BioTherapeutics, Inc. AGLE 0001707657 Souza Marcio C/O AEGLEA BIOTHERAPEUTICS, INC. 805 LAS CIMAS PARKWAY, #100 AUSTIN TX 78746 1 0 0 0 Director Stock Option (right to buy) 0.73 2022-06-07 4 A 0 43000 0.00 A 2032-06-06 Common Stock 43000 43000 D The stock option vests and becomes exercisable in 12 equal monthly installments beginning on July 7, 2022, or on the date of the next annual meeting of stockholders, if earlier, subject to the continuing service of the Reporting Person on each vesting date. Exhibit 24 - Power of Attorney /s/ James Kastenmayer, by power of attorney 2022-06-09