X0407 4 2023-07-05 0001703647 Frequency Therapeutics, Inc. FREQ 0001785288 Lucchino David L. C/O FREQUENCY THERAPEUTICS, INC. 75 HAYDEN AVE, SUITE 300 LEXINGTON MA 02421 1 1 President and CEO 0 Common Stock 2023-07-05 4 M 0 9091 A 432512 D Common Stock 2023-07-06 4 S 0 2773 .34 D 429739 D Common Stock 2023-07-06 4 M 0 9091 A 438830 D Restricted Stock Unit 2023-07-05 4 M 0 9091 D Common Stock 9091 290909 D Restricted Stock Unit 2023-07-06 4 M 0 9091 D Common Stock 9091 281818 D Each restricted stock unit represents a contingent right to receive on share of Issuer common stock. Represents a portion of the shares required to be sold by the Reporting Person to cover tax withholding obligations in connection with the vesting of the restricted stock units pursuant to a Rule 10b5-1 trading plan entered into on March 22, 2022 and does not represent discretionary trades by the Reporting Person. This transaction was executed in multiple trades through a broker-dealer at prices ranging from $0.33 to $0.35. The price reported in this column reflects the weighted average sales price. Upon request, the reporting person will provide to the SEC staff full information regarding the number of Shares sold at each price. The restricted stock units will fully vest on July 4, 2023. /s/ James P. Abely, Attorney-in-Fact for David L. Lucchino 2023-07-07