Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in the Registration Statements on Form S-3 (File No. 333-268341) and Form S-8 (No. 333-270393, No. 333-236170, 333-254686 and 333-263648) of Black Diamond Therapeutics, Inc. of our report dated March 12, 2024 relating to the financial statements, which appears in this Form 10-K.

/s/ PricewaterhouseCoopers LLP

Boston, Massachusetts

March 12, 2024