<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/schedule13g" xmlns:com="http://www.sec.gov/edgar/common">
  <headerData>
    <submissionType>SCHEDULE 13G</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0002113205</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
  </headerData>
  <formData>
    <coverPageHeader>
      <securitiesClassTitle>Common Stock, par value $4.00 per share</securitiesClassTitle>
      <eventDateRequiresFilingThisStatement>12/31/2025</eventDateRequiresFilingThisStatement>
      <issuerInfo>
        <issuerCik>0001693577</issuerCik>
        <issuerName>MainStreet Bancshares, Inc.</issuerName>
        <issuerCusip>56064Y100</issuerCusip>
        <issuerPrincipalExecutiveOfficeAddress>
          <com:street1>10089 FAIRFAX BOULEVARD</com:street1>
          <com:city>FAIRFAX</com:city>
          <com:stateOrCountry>VA</com:stateOrCountry>
          <com:zipCode>22030</com:zipCode>
        </issuerPrincipalExecutiveOfficeAddress>
      </issuerInfo>
      <designateRulesPursuantThisScheduleFiled>
        <designateRulePursuantThisScheduleFiled>Rule 13d-1(b)</designateRulePursuantThisScheduleFiled>
      </designateRulesPursuantThisScheduleFiled>
    </coverPageHeader>
    <coverPageHeaderReportingPersonDetails>

      <reportingPersonName>MainStreet Bank 401(k) Retirement Plan</reportingPersonName>
      <citizenshipOrOrganization>VA</citizenshipOrOrganization>
      <reportingPersonBeneficiallyOwnedNumberOfShares>
        <soleVotingPower>0.00</soleVotingPower>
        <sharedVotingPower>383763.00</sharedVotingPower>
        <soleDispositivePower>0.00</soleDispositivePower>
        <sharedDispositivePower>383763.00</sharedDispositivePower>
      </reportingPersonBeneficiallyOwnedNumberOfShares>
      <reportingPersonBeneficiallyOwnedAggregateNumberOfShares>383763.00</reportingPersonBeneficiallyOwnedAggregateNumberOfShares>
      <classPercent>5.1</classPercent>
      <typeOfReportingPerson>EP</typeOfReportingPerson>
      <comments>MainStreet Bancshares, Inc. on behalf of MainStreet Bank 401(k) Retirement Plan</comments>
    </coverPageHeaderReportingPersonDetails>
    <items>
      <item1>
        <issuerName>MainStreet Bancshares, Inc.</issuerName>
        <issuerPrincipalExecutiveOfficeAddress>10089 FAIRFAX BOULEVARD, FAIRFAX, VIRGINIA, 22030.</issuerPrincipalExecutiveOfficeAddress>
      </item1>
      <item2>
        <filingPersonName>Richard A. Vari, as Administrator for the Plan</filingPersonName>
        <principalBusinessOfficeOrResidenceAddress>10089 Fairfax Boulevard, Fairfax, VA 22030</principalBusinessOfficeOrResidenceAddress>
        <citizenship>Virginia</citizenship>
      </item2>
      <item3>
        <notApplicableFlag>N</notApplicableFlag>
        <typeOfPersonFiling>EP</typeOfPersonFiling>
      </item3>
      <item4>
        <amountBeneficiallyOwned>383,763</amountBeneficiallyOwned>
        <classPercent>5.1</classPercent>
        <numberOfSharesPersonHas>
          <solePowerOrDirectToVote>0</solePowerOrDirectToVote>
          <sharedPowerOrDirectToVote>383,763

The Plan is subject to the Employee Retirement Income Security Act of 1974 ("ERISA"). Delaware Charter Guarantee &amp; Trust Company dba Principal Trust Company acts as Trustee of the Plan (the "Trust"). The Trust is subject to fiduciary duties under ERISA and the Trust agreements.

Before each meeting of shareholders, Plan participants may direct the Trustee how to vote at such meeting or any adjournment thereof. If participants have not directed the Trustee on how to vote, the Trustee shall vote such securities in the same proportion as those shares for which the Trustee has received proper direction.</sharedPowerOrDirectToVote>
          <solePowerOrDirectToDispose>0</solePowerOrDirectToDispose>
          <sharedPowerOrDirectToDispose>383,763</sharedPowerOrDirectToDispose>
        </numberOfSharesPersonHas>
      </item4>
      <item5>
        <notApplicableFlag>Y</notApplicableFlag>
        <classOwnership5PercentOrLess>N</classOwnership5PercentOrLess>
      </item5>
      <item6>
        <notApplicableFlag>Y</notApplicableFlag>
      </item6>
      <item7>
        <notApplicableFlag>Y</notApplicableFlag>
      </item7>
      <item8>
        <notApplicableFlag>Y</notApplicableFlag>
      </item8>
      <item9>
        <notApplicableFlag>Y</notApplicableFlag>
      </item9>
      <item10>
        <notApplicableFlag>N</notApplicableFlag>
        <certifications>By signing below I certify that, to the best of my knowledge and belief, the foreign regulatory scheme applicable to Employee Benefit Plan is substantially comparable to the regulatory scheme applicable to the functionally equivalent U.S. institution(s). I also undertake to furnish to the Commission staff, upon request, information that would otherwise be disclosed in a Schedule 13D.

</certifications>
      </item10>
    </items>
    <signatureInformation>
      <reportingPersonName>MainStreet Bank 401(k) Retirement Plan</reportingPersonName>
      <signatureDetails>
        <signature>/s/ Richard A. Vari</signature>
        <title>Richard A. Vari/Plan Administrator</title>
        <date>02/24/2026</date>
      </signatureDetails>
    </signatureInformation>
  </formData>
</edgarSubmission>
