<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2020-07-23</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001693011</issuerCik>
        <issuerName>Inozyme Pharma, Inc.</issuerName>
        <issuerTradingSymbol>INZY</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001389517</rptOwnerCik>
            <rptOwnerName>Huertas Pedro</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O INOZYME PHARMA, INC.</rptOwnerStreet1>
            <rptOwnerStreet2>321 SUMMER STREET, SUITE 400</rptOwnerStreet2>
            <rptOwnerCity>BOSTON</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02210</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>See Remarks</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Stock Option (right to buy)</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>2.02</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F1"/>
            </exerciseDate>
            <expirationDate>
                <value>2029-09-25</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>160578</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">This option was granted on September 26, 2019. The shares underlying the option vested with respect to 25% of the shares on September 26, 2020 and the remainder are scheduled to vest in equal monthly installments through September 26, 2023.</footnote>
    </footnotes>

    <remarks>Senior Vice President, Chief Medical Officer

Exhibit Index: 24.1 Power of Attorney</remarks>

    <ownerSignature>
        <signatureName>/s/ Stephen Basso, as attorney-in-fact for Pedro Huertas</signatureName>
        <signatureDate>2020-07-23</signatureDate>
    </ownerSignature>
</ownershipDocument>
