<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2019-10-23</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001288750</issuerCik>
        <issuerName>Timberline Resources Corp</issuerName>
        <issuerTradingSymbol>TLRS</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001692272</rptOwnerCik>
            <rptOwnerName>Matlack William Fuller</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O TIMBERLINE RESOURCES CORPORATION</rptOwnerStreet1>
            <rptOwnerStreet2>101 EAST LAKESIDE AVENUE</rptOwnerStreet2>
            <rptOwnerCity>COEUR D'ALENE</rptOwnerCity>
            <rptOwnerState>ID</rptOwnerState>
            <rptOwnerZipCode>83814</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>13021679</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Series D Warrants</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>0.14</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2018-09-09</value>
                <footnoteId id="F1"/>
            </exerciseDate>
            <expirationDate>
                <value>2021-04-30</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common shares</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>2025000</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Series I Warrants</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>0.07</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2019-11-09</value>
                <footnoteId id="F1"/>
            </exerciseDate>
            <expirationDate>
                <value>2020-11-07</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common shares</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>3543600</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Series J Warrants</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>0.12</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2020-02-24</value>
                <footnoteId id="F1"/>
            </exerciseDate>
            <expirationDate>
                <value>2024-10-15</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common shares</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>3562500</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Series K Warrants</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>0.08</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2020-04-08</value>
                <footnoteId id="F1"/>
            </exerciseDate>
            <expirationDate>
                <value>2023-01-20</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common shares</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>4000000</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Stock options</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>0.08</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2019-10-29</value>
            </exerciseDate>
            <expirationDate>
                <value>2024-10-29</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common shares</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>404348</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Series E Warrants</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>0.08</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2019-03-09</value>
                <footnoteId id="F1"/>
            </exerciseDate>
            <expirationDate>
                <value>2021-10-29</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common shares</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>3678979</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">Warrants may not be exercised, except within 61 days of expiration, if such exercise would cause holder to beneficially own or exercise control or direction over 9.99% or greater of the issued and outstanding voting shares of the issuer. Warrants may not be exercised if such exercise would cause holder to beneficially own or exercise control or direction over 19.99% or greater of the issued and outstanding voting shares of the issuer.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>William Matlack</signatureName>
        <signatureDate>2020-01-10</signatureDate>
    </ownerSignature>
</ownershipDocument>
