<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2016-10-27</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001666134</issuerCik>
        <issuerName>BLACKLINE, INC.</issuerName>
        <issuerTradingSymbol>BL</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001688124</rptOwnerCik>
            <rptOwnerName>Griffith William J.G.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O ICONIQ STRATEGIC PARTNERS</rptOwnerStreet1>
            <rptOwnerStreet2>394 PACIFIC AVENUE, 2ND FLOOR</rptOwnerStreet2>
            <rptOwnerCity>SAN FRANCISCO</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94111</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>9428570</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See Footnotes</value>
                    <footnoteId id="F1"/>
                    <footnoteId id="F2"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">The shares are held by ICONIQ Strategic Partners, L.P. (&quot;ICONIQ&quot;), ICONIQ Strategic Partners-B, L.P. (&quot;ICONIQ B&quot;), ICONIQ Strategic Partners Co-Invest, L.P., BL Series (&quot;ICONIQ BL&quot;) and  ICONIQ Strategic Partners Co-Invest, L.P., BL 2 Series (&quot;ICONIQ BL2&quot;) (collectively, the &quot;ICONIQ Shares&quot;).  Iconiq Strategic Partners GP, L.P. (the &quot;ICONIQ GP&quot;), is the general partner of each of ICONIQ, ICONIQ B, ICONIQ BL and ICONIQ BL2.  ICONIQ Strategic Partners TT GP, Ltd. (the &quot;ICONIQ Parent GP&quot;) is the general partner of the ICONIQ GP.</footnote>
        <footnote id="F2">(Continued from Footnote 1) The Reporting Person is an equity holder and director of ICONIQ Parent GP and disclaims beneficial ownership of the ICONIQ Shares for purposes of Section16 of the Securities Exchange Act of 1934, as amended (the &quot;Exchange Act&quot;), except to the extent of his pecuniary interest therein, if any. This report shall not be deemed an admission that the Reporting Person is a beneficial owner of such securities for the purpose of Section 16 of the Exchange Act or for any other purpose.</footnote>
    </footnotes>

    <remarks>Exhibit 24.1 - Power of Attorney</remarks>

    <ownerSignature>
        <signatureName>/s/ Karole Morgan-Prager, Attorney-in-Fact</signatureName>
        <signatureDate>2016-10-27</signatureDate>
    </ownerSignature>
</ownershipDocument>
