144 0001388325 XXXXXXXX LIVE 0001683553 SPRUCE BIOSCIENCES, INC. 001-39594 611 GATEWAY BOULEVARD, SUITE 740 SOUTH SAN FRANCISCO CA 94080 (415) 655-4168 Novo Holdings A/S 10% Stockholder Common Stock Morgan Stanley & Co. LLC

1585 Broadway New York NY 10036

1912316 9485087.36 41029832 03/14/2024 NASDAQGS Common Stock 10/14/2020 Conversion of Series A Preferred Stock in Initial Public Offering Spruce Biosciences, Inc. N 3210518 10/14/2020 Conversion of Preferred Stock Y 03/14/2024 /s/ Barbara Fiorini Due, General Counsel, Finance & Operations