<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2023-09-11</periodOfReport>

    <issuer>
        <issuerCik>0001679063</issuerCik>
        <issuerName>Chicken Soup for the Soul Entertainment, Inc.</issuerName>
        <issuerTradingSymbol>CSSE</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0000904523</rptOwnerCik>
            <rptOwnerName>ROUHANA WILLIAM J JR</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O CHICKEN SOUP FOR THE SOUL ENTERTAINM</rptOwnerStreet1>
            <rptOwnerStreet2>132 E. PUTNAM AVENUE, FL. 2</rptOwnerStreet2>
            <rptOwnerCity>COS COB</rptOwnerCity>
            <rptOwnerState>CT</rptOwnerState>
            <rptOwnerZipCode>06807</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>Chief Executive Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001714518</rptOwnerCik>
            <rptOwnerName>Chicken Soup for the Soul Holdings, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O CHICKEN SOUP FOR THE SOUL ENTERTAINM</rptOwnerStreet1>
            <rptOwnerStreet2>132 E. PUTNAM AVENUE, FL. 2</rptOwnerStreet2>
            <rptOwnerCity>COS COB</rptOwnerCity>
            <rptOwnerState>CT</rptOwnerState>
            <rptOwnerZipCode>06807</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001714509</rptOwnerCik>
            <rptOwnerName>Chicken Soup for the Soul Productions, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O CHICKEN SOUP FOR THE SOUL ENTERTAINM</rptOwnerStreet1>
            <rptOwnerStreet2>132 E. PUTNAM AVENUE, FL. 2</rptOwnerStreet2>
            <rptOwnerCity>COS COB</rptOwnerCity>
            <rptOwnerState>CT</rptOwnerState>
            <rptOwnerZipCode>06807</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Class A Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2023-09-11</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>J</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
                <footnoteId id="F1"/>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>403799</value>
                    <footnoteId id="F1"/>
                </transactionShares>
                <transactionPricePerShare>
                    <footnoteId id="F1"/>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>3688070</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>by Chicken Soup for the Soul, LLC</value>
                    <footnoteId id="F2"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Class A Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>510108</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>by Trema, LLC</value>
                    <footnoteId id="F3"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Class B Common Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F4"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F4"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F4"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Class A Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>7654506</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>7654506</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>by Chicken Soup for the Soul, LLC</value>
                    <footnoteId id="F2"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">Represents shares of the Issuer's Class A common stock issued to Chicken Soup for the Soul, LLC pursuant to the CSS Agreements Modification dated March 30, 2023.</footnote>
        <footnote id="F2">Chicken Soup for the Soul, LLC, immediate parent of the Issuer, is an entity controlled by reporting person, William J. Rouhana, Jr., the Chief Executive Officer and a Director of the Issuer. This entity was formerly known as Chicken Soup for the Soul Productions, LLC. Chicken Soup for the Soul, LLC is owned by Chicken Soup for the Soul Holdings, LLC, which is also an entity controlled by Mr. Rouhana.</footnote>
        <footnote id="F3">Trema, LLC is an entity controlled by Mr. Rouhana.</footnote>
        <footnote id="F4">The Class B Common Stock is convertible into the Issuer's Class A Common Stock as described under the heading &quot;Description of Securities - Common Stock&quot; in the Issuer's offering statement on Form 1-A (File No. 024-10704). The Class B Common Stock has no expiration date.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>William J. Rouhana, Jr.</signatureName>
        <signatureDate>2023-09-12</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>William J. Rouhana, Jr., as managing member of Chicken Soup for the Soul, LLC fka Chicken Soup for the Soul Productions, LLC</signatureName>
        <signatureDate>2023-09-12</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>William J. Rouhana, Jr., as managing member of Chicken Soup for the Soul Holdings, LLC</signatureName>
        <signatureDate>2023-09-12</signatureDate>
    </ownerSignature>
</ownershipDocument>
