X0306 4 2021-12-13 0 0001678660 Prelude Therapeutics Inc PRLD 0001329010 Chardonnet Laurent C/O PRELUDE THERAPEUTICS INCORPORATED 200 POWDER MILL ROAD WILMINGTON DE 19801 0 1 0 0 Chief Financial Officer Common Stock 2021-12-13 4 A 0 5000 12.28 A 8000 D The price reported in Column 4 is a weighted average price. These shares were purchased in multiple transactions at prices ranging from $12.24 to $12.29, inclusive. The reporting person undertakes to provide to the Issuer, any security holder of the Issuer, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares purchased at each separate price within the ranges set forth herein. /s/ Krishna Vaddi, Attorney-in-Fact 2021-12-14