X0306 4 2020-09-29 0 0001678660 Prelude Therapeutics Inc PRLD 0001824633 Vaddi Krishna C/O PRELUDE THERAPEUTICS INCORPORATED 200 POWDER MILL ROAD WILMINGTON DE 19801 1 1 0 0 President, CEO Common Stock 1045519 D Common Stock 2020-09-29 4 C 0 101477 A 101477 I See Footnote Common Stock 2020-09-29 4 C 0 32876 A 551776 I By Trust Common Stock 2020-09-29 4 C 0 32876 A 551774 I By Trust Common Stock 423655 I By Trust Series A Preferred Stock 2020-09-29 4 C 0 101477 0.00 D Common Stock 101477 0 I See Footnote Series A Preferred Stock 2020-09-29 4 C 0 32876 0.00 D Common Stock 32876 0 I By Trust Series A Preferred Stock 2020-09-29 4 C 0 32876 0.00 D Common Stock 32876 0 I By Trust Certain of the shares are subject to forfeiture to the Issuer if underlying vesting conditions are not met. The Series A Preferred Stock automatically converted into shares of the Issuer's common stock on a one-for-one basis immediately upon the closing of the Issuer's initial public offering on September 29, 2020 and had no expiration date. The Reporting Person is the manager of Sidus Ventures LLC. The Reporting Person disclaims beneficial ownership of such securities except to the extent of his pecuniary interest therein, and the inclusion of these securities in this report shall not be deemed an admission of beneficial ownership of such securities for purposes of Section 16 or for any other purposes. The Reporting Person is the investment advisor of the Blue Sky Trust. The Reporting Person disclaims beneficial ownership of such securities except to the extent of his pecuniary interest therein, and the inclusion of these securities in this report shall not be deemed an admission of beneficial ownership of such securities for purposes of Section 16 or for any other purposes. The Reporting Person is the investment advisor of the Brocade Trust. The Reporting Person disclaims beneficial ownership of such securities except to the extent of his pecuniary interest therein, and the inclusion of these securities in this report shall not be deemed an admission of beneficial ownership of such securities for purposes of Section 16 or for any other purposes. The Reporting Person is a beneficiary of the Dolphin City Trust. The Reporting Person disclaims beneficial ownership of such securities except to the extent of his pecuniary interest therein, and the inclusion of these securities in this report shall not be deemed an admission of beneficial ownership of such securities for purposes of Section 16 or for any other purposes. /s/ Brian Piper, as Attorney-in-Fact for Krishna Vaddi 2020-09-29