X0206 3 2020-09-24 0 0001678660 Prelude Therapeutics Inc PRLD 0001824625 Morosini Deborah C/O PRELUDE THERAPEUTICS INCORPORATED 200 POWDER MILL ROAD WILMINGTON DE 19801 0 1 0 0 EVP, Chief of Clinical Affairs Employee Stock Option (right to buy) 1.89 2030-03-26 Common Stock 8646 D Employee Stock Option (right to buy) 12.85 2030-09-01 Common Stock 99428 D Employee Stock Option (right to buy) 12.85 2030-09-01 Common Stock 224796 D The stock option vests as to 1/12 of the total shares monthly beginning on December 18, 2019. The stock option vests as to 25% of the total shares on September 24, 2021, and thereafter vests as to 1/48 of the total share monthly until fully vested, subject to the Reporting Person's provision of service to the Issuer on each vesting date. The stock option vests as to 25% of the total shares on July 27, 2020, and thereafter vests as to 1/48 of the total shares monthly until fully vested, subject to the Reporting Person's provision of service to the Issuer on each vesting date. /s/ Brian Piper, as Attorney-in-Fact for Deborah Morosini 2020-09-24