X0607 3 2026-03-05 0 0001678660 Prelude Therapeutics Inc PRLD 0001884733 Brusky Sean P. C/O PRELUDE THERAPEUTICS INCORPORATED 175 INNOVATION BOULEVARD WILMINGTON DE 19805 true Chief Business Officer Common Stock 100000 D Employee Stock Option (Right to Buy) 0 2034-04-30 Common Stock 300000 D Employee Stock Option (Right to Buy) 0 2035-02-03 Common Stock 175000 D Employee Stock Option (Right to Buy) 0 2036-02-03 Common Stock 275000 D Stock option grant awarded on May 1, 2024 under the 2020 Equity Incentive Plan. The stock option vests as to 25% of the total shares on May 1, 2025, and thereafter vests as to 1/48th of the total shares monthly until fully vested, subject to the Reporting Person's provision of service to the Issuer on each vesting date. Stock option grant awarded on February 4, 2025 under the 2020 Equity Incentive Plan. The stock option vests as to 25% of the total shares on February 4, 2026, and thereafter vests as to 1/48th of the total shares monthly until fully vested, subject to the Reporting Person's provision of service to the Issuer on each vesting date. Stock option grant awarded on February 4, 2026 under the 2020 Equity Incentive Plan. The stock option vests as to 25% of the total shares on February 4, 2027, and thereafter vests as to 1/48th of the total shares monthly until fully vested, subject to the Reporting Person's provision of service to the Issuer on each vesting date. Exhibit 24 - Power of Attorney /s/ Bryant Lim, as attorney-in-fact for the Reporting Person 2026-03-19