X0508 4 2023-11-15 0001625101 Pulse Biosciences, Inc. PLSE 0001674465 Levinson Mitchell E. 3957 POINT EDEN WAY HAYWARD CA 94545 1 Chief Strategy Officer 0 Common Stock 2023-11-15 4 P 0 461 5.832 A 69227 D Common Stock 2023-11-15 4 P 0 3800 5.88 A 73027 D Common Stock 2023-11-15 4 P 0 5000 5.9399 A 9135 I Spouse Common Stock 2023-11-15 4 P 0 200 5.92 A 934 I Immediate Family Member (1) Common Stock 2023-11-15 4 P 0 2300 5.9399 A 3234 I Immediate Family Member (1) Common Stock 2023-11-15 4 P 0 100 5.94 A 834 I Immediate Family Member (2) Common Stock 2023-11-15 4 P 0 2400 5.8457 A 3234 I Immediate Family Member (2) Common Stock 2023-11-15 4 P 0 100 5.90 A 834 I Immediate Family Member (3) Common Stock 2023-11-15 4 P 0 2400 5.80260 A 3234 I Immediate Family Member (3) Common Stock 2023-11-15 4 P 0 1039 6.1332 A 1039 I Immediate family member (4) /s/ Kenneth B. Stratton, as Attorney-in-Fact 2023-11-17