X0407 4 2022-03-22 0001625101 Pulse Biosciences, Inc. PLSE 0001674465 Levinson Mitchell E. 3957 POINT EDEN WAY HAYWARD CA 94545 1 Chief Strategy Officer 0 Stock Option (right to buy) 2.92 2023-03-22 4 A 0 35000 A 2033-03-22 Common Stock 35000 35000 D The option was granted on March 22, 2023 under the Issuer's 2017 Equity Incentive Plan. The shares subject to the option are subject to performance-based vesting criteria. 8,750 shares will vest in equal annual installments over the following four years on or about December 31, starting in 2023, subject to achievement of the vesting criteria and the reporting person's continued service through each vesting date. Not Applicable /s/ Kenneth B. Stratton, as Attorney-in-Fact 2023-03-24