<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0707</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001668159</cik>
        <entityName>COLUMN GROUP III, LP</entityName>
        <issuerAddress>
            <street1>1700 OWENS STREET</street1>
            <street2>SUITE 500</street2>
            <city>SAN FRANCISCO</city>
            <stateOrCountry>CA</stateOrCountry>
            <stateOrCountryDescription>CALIFORNIA</stateOrCountryDescription>
            <zipCode>94158</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>4158652050</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Limited Partnership</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2016</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>DAVID</firstName>
                <middleName>V</middleName>
                <lastName>GOEDDEL PH D</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>1700 OWENS STREET</street1>
                <street2>SUITE 500</street2>
                <city>SAN FRANCISCO</city>
                <stateOrCountry>CA</stateOrCountry>
                <stateOrCountryDescription>CALIFORNIA</stateOrCountryDescription>
                <zipCode>94158</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>PETER</firstName>
                <lastName>SVENNILSON</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>1700 OWENS STREET</street1>
                <street2>SUITE 500</street2>
                <city>SAN FRANCISCO</city>
                <stateOrCountry>CA</stateOrCountry>
                <stateOrCountryDescription>CALIFORNIA</stateOrCountryDescription>
                <zipCode>94158</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>TIM</firstName>
                <lastName>KUTZKEY</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>1700 OWENS STREET</street1>
                <street2>SUITE 500</street2>
                <city>SAN FRANCISCO</city>
                <stateOrCountry>CA</stateOrCountry>
                <stateOrCountryDescription>CALIFORNIA</stateOrCountryDescription>
                <zipCode>94158</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Pooled Investment Fund</industryGroupType>
            <investmentFundInfo>
                <investmentFundType>Venture Capital Fund</investmentFundType>
                <is40Act>false</is40Act>
            </investmentFundInfo>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
            <item>3C</item>
            <item>3C.1</item>
            <item>3C.7</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001668159-16-000001</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2016-04-01</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
            <isPooledInvestmentFundType>true</isPooledInvestmentFundType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>25000</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>239535000</totalOfferingAmount>
            <totalAmountSold>239535000</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse>THE GENERAL PARTNER OF THE ISSUER RESERVES THE RIGHT TO OFFER A GREATER OR LESSER AMOUNT OF LIMITED PARTNER INTERESTS. THE FIGURE IN ITEM 13(a)INCLUDES AMOUNTS IN RESPECT OF THE ISSUER'S PARALLEL ENTITIES.</clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>39</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse>THE GENERAL PARTNER IS ENTITLED TO A CARRIED INTEREST.  THE INVESTMENT MANAGER IS ENTITLED TO A MANAGEMENT FEE.  THE CARRIED INTEREST AND MANAGEMENT FEE ARE FULLY DISCUSSED IN THE ISSUER?S CONFIDENTIAL OFFERING MATERIALS.</clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>COLUMN GROUP III, LP</issuerName>
                <signatureName>/s/JAMES EVANGELISTA</signatureName>
                <nameOfSigner>JAMES EVANGELISTA</nameOfSigner>
                <signatureTitle>CHIEF FINANCIAL OFFICER</signatureTitle>
                <signatureDate>2016-04-27</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
