SCHEDULE 13G 0001482416 XXXXXXXX LIVE Common stock, par value $0.0001 per share 03/31/2025 0001661998 Q32 Bio Inc. 746964105 830 WINTER STREET WALTHAM MA 02451 Rule 13d-1(b) Sio Capital Management, LLC DE 0.00 0.00 0.00 0.00 0.00 N 0 IA Q32 Bio Inc. 830 WINTER STREET, WALTHAM, MASSACHUSETTS, 02451 This Statement is filed on behalf of Sio Capital Management, LLC (the "Reporting Person" or "Sio") Sio is a registered investment adviser to certain affiliated funds that directly hold the shares of Common Stock to which this statement relates for the benefit of their respective investors, and in such capacity Sio has voting and dispositive power over such shares. 600 Third Avenue, 2nd Floor New York, New York 10016 Sio is a Delaware limited liability company. N IA -0- 0% 0 0 0 0 N Y Y Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were acquired and are held in the ordinary course of business and were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. Sio Capital Management, LLC /s/ Jin W. Lee 04/04/2025