X0202 SCHEDULE 13G/A 0000902664-26-001202 0001603466 XXXXXXXX LIVE 2 Common Stock, par value $0.0001 per share 06/30/2026 0001661998 Q32 Bio Inc. 746964105 830 WINTER STREET WALTHAM MA 02451 Rule 13d-1(c) Point72 Asset Management, L.P. b DE 0.00 1173396.00 0.00 1173396.00 1173396.00 N 4.95 PN Point72 Capital Advisors, Inc. b DE 0.00 1173396.00 0.00 1173396.00 1173396.00 N 4.95 CO Steven A. Cohen b X1 0.00 1173396.00 0.00 1173396.00 1173396.00 N 4.95 IN Q32 Bio Inc. 830 WINTER STREET, WALTHAM, MA 02451 This statement is filed by: (i) Point72 Asset Management, L.P. ("Point72 Asset Management") with respect to the shares of common stock, par value $0.0001 per share ("Common Stock"), of Q32 Bio Inc. (the "Issuer") held by an investment fund it manages; (ii) Point72 Capital Advisors, Inc. ("Point72 Capital Advisors Inc.") with respect to the shares of Common Stock held by an investment fund managed by Point72 Asset Management; and (iii) Steven A. Cohen ("Mr. Cohen") with respect to the shares of Common Stock beneficially owned by Point72 Asset Management and Point72 Capital Advisors Inc. Cubist Systematic Strategies, LLC is a relying adviser on the Form ADV of Point72 Asset Management and acts as a sub-advisor with respect to a portion of the shares of Common Stock reported herein. The address of the principal business office of Point72 Asset Management, Point72 Capital Advisors Inc., and Mr. Cohen is 72 Cummings Point Road, Stamford, CT 06902. Point72 Asset Management is a Delaware limited partnership. Point72 Capital Advisors Inc. is a Delaware corporation. Mr. Cohen is a United States citizen. Y The information required by Item 4(a) is set forth in Row 9 of the cover page for each of the Reporting Persons and is incorporated herein by reference. Such information is as of the close of business on June 30, 2026. Point72 Asset Management, Point72 Capital Advisors Inc., and Mr. Cohen own directly no shares of Common Stock. Pursuant to an investment management agreement, Point72 Asset Management maintains investment and voting power with respect to the securities held by an investment fund it manages. Point72 Capital Advisors Inc. is the general partner of Point72 Asset Management. Mr. Cohen controls each of Point72 Asset Management and Point72 Capital Advisors Inc. The filing of this statement should not be construed as an admission that any of the foregoing persons or any Reporting Person is, for the purposes of Section 13 of the Securities Exchange Act of 1934, the beneficial owner of the shares of Common Stock reported herein. The percentages set forth in this Schedule 13G are calculated based upon an aggregate of 23,681,415 shares of Common Stock outstanding, which is the sum of (i) 16,956,415 shares of Common Stock outstanding as of May 26, 2026, as reported in Exhibit 10.1 attached to the Issuer's Current Report on Form 8-K filed with the Securities and Exchange Commission on May 27, 2026 (the "May 27th 8-K"); and (ii) 6,725,000 shares of Common Stock issued in the private placement entered into on May 26, 2026, as described in the May 27th 8-K. 4.95% The information required by Item 4(c)(i) is set forth in Row 5 of the cover page for each of the Reporting Persons and is incorporated herein by reference. Such information is as of the close of business on June 30, 2026. The information required by Item 4(c)(ii) is set forth in Row 6 of the cover page for each of the Reporting Persons and is incorporated herein by reference. Such information is as of the close of business on June 30, 2026. The information required by Item 4(c)(iii) is set forth in Row 7 of the cover page for each of the Reporting Persons and is incorporated herein by reference. Such information is as of the close of business on June 30, 2026. The information required by Item 4(c)(iv) is set forth in Row 8 of the cover page for each of the Reporting Persons and is incorporated herein by reference. Such information is as of the close of business on June 30, 2026. N Y N See Item 2(a). Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under § 240.14a-11. Point72 Asset Management, L.P. /s/ Jason M. Colombo 08/14/2026 Point72 Capital Advisors, Inc. /s/ Jason M. Colombo 08/14/2026 Steven A. Cohen /s/ Jason M. Colombo 08/14/2026