Exhibit 21.1

SUBSIDIARIES

Amylyx Pharmaceuticals, Inc. had international subsidiaries shown below as of February 22, 2024. Amylyx Pharmaceuticals, Inc. is not a subsidiary of any other entity.

Subsidiary	Jurisdiction of Incorporation
Amylyx Pharmaceuticals Canada Inc.	Canada
Amylyx Pharmaceuticals EMEA B.V.	Netherlands
Amylyx Pharmaceuticals Distribution Ltd.	Ireland
Amylyx Pharmaceuticals Germany GmbH	Germany
Amylyx Pharmaceuticals France SAS	France
Amylyx Pharmaceuticals Belgium SRL	Belgium
Amylyx Pharmaceuticals UK Ltd.	United Kingdom
Amylyx Pharmaceuticals Japan K.K.	Japan