Exhibit 21.1

SUBSIDIARIES

Amylyx Pharmaceuticals, Inc. had international subsidiaries shown below as of March 13, 2023. Amylyx Pharmaceuticals, Inc. is not a subsidiary of any other entity.

Subsidiary	Jurisdiction of Incorporation
Amylyx Pharmaceuticals Canada Inc.	Canada
Amylyx Pharmaceuticals EMEA B.V.	Netherlands
Amylyx Pharmaceuticals Distribution Ltd.	Ireland
Amylyx Pharmaceuticals Germany GmbH	Germany
Amylyx Pharmaceuticals France SAS	France