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    <filerInfo>
      <liveTestFlag>LIVE</liveTestFlag>
      <filer>
        <issuerCredentials>
          <cik>0001656367</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
        <offeringFileNumber>024-11720</offeringFileNumber>
      </filer>
      <flags>
        <returnCopyFlag>false</returnCopyFlag>
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        <sinceLastFiling>false</sinceLastFiling>
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    <employeesInfo>
      <issuerName>ClearLight Biotechnologies, Inc.</issuerName>
      <jurisdictionOrganization>DE</jurisdictionOrganization>
      <yearIncorporation>2014</yearIncorporation>
      <cik>0001656367</cik>
      <sicCode>8071</sicCode>
      <irsNum>85-3503126</irsNum>
      <fullTimeEmployees>5</fullTimeEmployees>
      <partTimeEmployees>0</partTimeEmployees>
    </employeesInfo>
    <issuerInfo>
      <street1>428 OAKMEAD PARKWAY</street1>
      <city>SUNNYVALE</city>
      <stateOrCountry>CA</stateOrCountry>
      <zipCode>94085</zipCode>
      <phoneNumber>8002518905</phoneNumber>
      <connectionName>Albert Hansen</connectionName>
      <industryGroup>Other</industryGroup>
      <cashEquivalents>718333.00</cashEquivalents>
      <investmentSecurities>0.00</investmentSecurities>
      <accountsReceivable>17595.00</accountsReceivable>
      <propertyPlantEquipment>818271.00</propertyPlantEquipment>
      <totalAssets>2072588.00</totalAssets>
      <accountsPayable>500080.00</accountsPayable>
      <longTermDebt>0.00</longTermDebt>
      <totalLiabilities>500080.00</totalLiabilities>
      <totalStockholderEquity>1572508.00</totalStockholderEquity>
      <totalLiabilitiesAndEquity>2072588.00</totalLiabilitiesAndEquity>
      <totalRevenues>29874.00</totalRevenues>
      <costAndExpensesApplToRevenues>157760.00</costAndExpensesApplToRevenues>
      <depreciationAndAmortization>31620.00</depreciationAndAmortization>
      <netIncome>-680461.00</netIncome>
      <earningsPerShareBasic>-0.73</earningsPerShareBasic>
      <earningsPerShareDiluted>-0.73</earningsPerShareDiluted>
    </issuerInfo>
    <commonEquity>
      <commonEquityClassName>Common Stock</commonEquityClassName>
      <outstandingCommonEquity>1134331</outstandingCommonEquity>
      <commonCusipEquity>N/A</commonCusipEquity>
      <publiclyTradedCommonEquity>N/A</publiclyTradedCommonEquity>
    </commonEquity>
    <preferredEquity>
      <preferredEquityClassName>Preferred Stock</preferredEquityClassName>
      <outstandingPreferredEquity>0</outstandingPreferredEquity>
      <preferredCusipEquity>N/A</preferredCusipEquity>
      <publiclyTradedPreferredEquity>N/A</publiclyTradedPreferredEquity>
    </preferredEquity>
    <debtSecurities>
      <debtSecuritiesClassName>None</debtSecuritiesClassName>
      <outstandingDebtSecurities>0</outstandingDebtSecurities>
      <cusipDebtSecurities>N/A</cusipDebtSecurities>
      <publiclyTradedDebtSecurities>N/A</publiclyTradedDebtSecurities>
    </debtSecurities>
    <issuerEligibility>
      <certifyIfTrue>true</certifyIfTrue>
    </issuerEligibility>
    <applicationRule262>
      <certifyIfNotDisqualified>true</certifyIfNotDisqualified>
      <certifyIfBadActor>false</certifyIfBadActor>
    </applicationRule262>
    <summaryInfo>
      <indicateTier1Tier2Offering>Tier1</indicateTier1Tier2Offering>
      <financialStatementAuditStatus>Unaudited</financialStatementAuditStatus>
      <securitiesOfferedTypes>Equity (common or preferred stock)</securitiesOfferedTypes>
      <offerDelayedContinuousFlag>Y</offerDelayedContinuousFlag>
      <offeringYearFlag>N</offeringYearFlag>
      <offeringAfterQualifFlag>N</offeringAfterQualifFlag>
      <offeringBestEffortsFlag>Y</offeringBestEffortsFlag>
      <solicitationProposedOfferingFlag>N</solicitationProposedOfferingFlag>
      <resaleSecuritiesAffiliatesFlag>N</resaleSecuritiesAffiliatesFlag>
      <securitiesOffered>20000</securitiesOffered>
      <outstandingSecurities>1134331</outstandingSecurities>
      <pricePerSecurity>12.0000</pricePerSecurity>
      <issuerAggregateOffering>240000.00</issuerAggregateOffering>
      <securityHolderAggegate>0.00</securityHolderAggegate>
      <qualificationOfferingAggregate>0.00</qualificationOfferingAggregate>
      <concurrentOfferingAggregate>0.00</concurrentOfferingAggregate>
      <totalAggregateOffering>240000.00</totalAggregateOffering>
      <underwritersFees>0.00</underwritersFees>
      <salesCommissionsServiceProviderFees>0.00</salesCommissionsServiceProviderFees>
      <finderFeesFee>0.00</finderFeesFee>
      <auditorFees>0.00</auditorFees>
      <legalServiceProviderName>Karen Muller, Esq.</legalServiceProviderName>
      <legalFees>35000.00</legalFees>
      <promotersFees>0.00</promotersFees>
      <blueSkyServiceProviderName>Karen Muller, Esq.</blueSkyServiceProviderName>
      <blueSkyFees>7240.00</blueSkyFees>
      <brokerDealerCrdNumber>0</brokerDealerCrdNumber>
      <estimatedNetAmount>197760.00</estimatedNetAmount>
      <clarificationResponses>Blue Sky Compliance may be lower, depending on the allocation of the offering in the jurisdictions in which the securities will be offered.</clarificationResponses>
    </summaryInfo>
    <juridictionSecuritiesOffered>
      <jurisdictionsOfSecOfferedNone>true</jurisdictionsOfSecOfferedNone>
      <jurisdictionsOfSecOfferedSame>false</jurisdictionsOfSecOfferedSame>
      <issueJuridicationSecuritiesOffering>CA</issueJuridicationSecuritiesOffering>
      <issueJuridicationSecuritiesOffering>CO</issueJuridicationSecuritiesOffering>
      <issueJuridicationSecuritiesOffering>FL</issueJuridicationSecuritiesOffering>
      <issueJuridicationSecuritiesOffering>MI</issueJuridicationSecuritiesOffering>
      <issueJuridicationSecuritiesOffering>NV</issueJuridicationSecuritiesOffering>
      <issueJuridicationSecuritiesOffering>NY</issueJuridicationSecuritiesOffering>
      <issueJuridicationSecuritiesOffering>TX</issueJuridicationSecuritiesOffering>
    </juridictionSecuritiesOffered>
    <unregisteredSecurities>
      <ifUnregsiteredNone>false</ifUnregsiteredNone>
    </unregisteredSecurities>
    <securitiesIssued>
      <securitiesIssuerName>ClearLight Biotechnologies, Inc.</securitiesIssuerName>
      <securitiesIssuerTitle>Common Stock</securitiesIssuerTitle>
      <securitiesIssuedTotalAmount>411381</securitiesIssuedTotalAmount>
      <securitiesPrincipalHolderAmount>0</securitiesPrincipalHolderAmount>
      <securitiesIssuedAggregateAmount>Total $2,310,012: 162,501 at $4 per share; 125,001 at $8 per share and 55,000 at $12 per share. Issuances on January 2, 2021 and January 29, 2021 were to employees and independent contractors under a plan.</securitiesIssuedAggregateAmount>
    </securitiesIssued>
    <unregisteredSecuritiesAct>
      <securitiesActExcemption>Section 4(a)(2) of the Securities Act.</securitiesActExcemption>
    </unregisteredSecuritiesAct>
  </formData>
</edgarSubmission>
